{"id":1678,"date":"2026-08-04T14:24:57","date_gmt":"2026-08-04T14:24:57","guid":{"rendered":""},"modified":"2026-08-04T23:15:40","modified_gmt":"2026-08-04T15:15:40","slug":"case-10-sample-live-actin-microtubule-sted-1678","status":"publish","type":"post","link":"https:\/\/www.hxicollege.com\/index.php\/2026\/08\/04\/case-10-sample-live-actin-microtubule-sted-1678\/","title":{"rendered":"\u6848\u4f8b10\uff1a\u9ad8\u4fdd\u771f\u8d85\u5206\u8fa8\u7ea7\u6837\u672c-\u7ec6\u80de\u5fae\u4e1d\u3001\u5fae\u7ba1\u6d3b\u7ec6\u80de STED \u6210\u50cf"},"content":{"rendered":"<p><!-- \u6848\u4f8b 10\uff1a\u9ad8\u4fdd\u771f\u8d85\u5206\u8fa8\u7ea7\u6837\u672c-\u7ec6\u80de\u5fae\u4e1d\u3001\u5fae\u7ba1\u6d3b\u7ec6\u80de STED \u6210\u50cf\n     \u5e03\u5c40 v1: \u878d\u5408\u6848\u4f8b 02 (post 1617) \u6d3b\u7ec6\u80de\u7248\u5f0f, \u5fae\u4e1d\u4e0e\u5fae\u7ba1\u53cc\u67d3 + STED \u6a21\u5f0f\n       1. CSS \u5185\u8054 (\u590d\u7528 case08)\n       2. \ud83d\udcfa \u89c6\u9891\u6309\u94ae\u7f6e\u9876 (\u6d3b\u7ec6\u80de STED \u6848\u4f8b\u901a\u5e38\u5e26\u89c6\u9891)\n       3. h2 \u6848\u4f8b\u6807\u9898\n       4. hx-grid \u4e24\u5217\n          \u5de6\u680f: hx-figure \u4e3b\u56fe\u5361 (\u9759\u6001 webp) + hx-protocol \u534f\u8bae\u5757\n          \u53f3\u680f: hx-block \u89e3\u8bfb\u94fe (\u56fe\u50cf\u63cf\u8ff0 \/ \u6807\u8bb0\u8981\u70b9 \/ \u6210\u50cf\u8981\u70b9 \/ \u6807\u8bb0\u4fe1\u606f \/ \u6210\u50cf\u8bbe\u5907)\n       5. hx-end \u6536\u5c3e\n     \u4e3b\u56fe\u5360\u4f4d\u7b26: https:\/\/www.hxicollege.com\n     \u534f\u8bae\u6807\u9898: Protocol for high-fidelity live Actin and Microtubule STED Sample\n     \u67d3\u8272\u901a\u9053\u5dee\u5f02: \u5fae\u4e1d=\u84dd\u8272, \u5fae\u7ba1=\u7d2b\u8272 (\u4e0e\u6848\u4f8b 08 \u84dd\u6a58\u53cd\u5411) --><\/p>\n<style>\n  \/* ===== \u6574\u7bc7\u5bb9\u5668 ===== *\/\n  .hx-article{\n    max-width:1080px;margin:0 auto;padding:40px 28px 64px;\n    font-family:-apple-system,BlinkMacSystemFont,\"Segoe UI\",\"PingFang SC\",\"Hiragino Sans GB\",\"Microsoft YaHei\",sans-serif;\n    color:#1f2328;line-height:1.85;font-size:16px;\n  }\n  .hx-article h2{font-size:22px;font-weight:800;color:#0f172a;margin:0 0 28px;line-height:1.4;}\n  \/* ===== \u89c6\u9891\u6309\u94ae ===== *\/\n  .hx-video-wrap{margin:8px 0 20px;display:flex;justify-content:center;}\n  .hx-video-btn{\n    display:inline-flex;align-items:center;gap:8px;\n    padding:12px 28px;border-radius:999px;\n    background:linear-gradient(135deg,#2563eb,#1d4ed8);\n    color:#fff !important;font-size:16px;font-weight:600;\n    text-decoration:none !important;\n    box-shadow:0 4px 14px rgba(37,99,235,.25),0 1px 3px rgba(15,23,42,.08);\n  }\n  \/* ===== 2 \u5217\u5e03\u5c40 ===== *\/\n  .hx-grid{display:flex;gap:26px;align-items:flex-start;}\n  .hx-grid .hx-col{flex:1 1 50%;min-width:0;}\n  \/* ===== \u5355\u56fe\u5361 ===== *\/\n  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li::before{content:counter(pc)\".\";position:absolute;left:0;top:0;color:#f97316;font-weight:800;}\n  .hx-protocol .k{background:rgba(37,99,235,.12);color:#2563eb;padding:1px 6px;border-radius:4px;font-weight:600;font-size:13px;}\n  .hx-protocol code{background:rgba(37,99,235,.12);color:#2563eb;padding:1px 6px;border-radius:4px;font-family:Menlo,Monaco,Consolas,monospace;font-size:.9em;}\n  \/* ===== \u53f3\u680f\u89e3\u8bfb\u5757 ===== *\/\n  .hx-block{margin-bottom:22px;}\n  .hx-block h4{font-size:15.5px;font-weight:800;color:#0f172a;margin:0 0 8px;display:flex;align-items:center;gap:6px;}\n  .hx-block p{margin:0 0 6px;color:#374151;font-size:15px;}\n  .hx-block ol{margin:4px 0 0;padding-left:20px;color:#374151;font-size:14.5px;}\n  .hx-block ol li{margin-bottom:5px;}\n  .hx-block .info-line{margin:2px 0;color:#374151;font-size:14.5px;}\n  .hx-block .info-line b{color:#0f172a;font-weight:600;}\n  \/* ===== \u98de\u4e66 ace-line \u517c\u5bb9 ===== *\/\n  .hx-block 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.hx-protocol li::before{color:#fb923c;}\n  body.aft-dark-mode .hx-protocol .k{background:rgba(37,99,235,.25);color:#93c5fd;}\n  body.aft-dark-mode .hx-protocol code{background:rgba(37,99,235,.25);color:#93c5fd;}\n  body.aft-dark-mode .hx-block h4{color:#f1f5f9;}\n  body.aft-dark-mode .hx-block p,body.aft-dark-mode .hx-block ol,body.aft-dark-mode .hx-block ol li{color:#cbd5e1;}\n  body.aft-dark-mode .hx-block .info-line{color:#cbd5e1;}\n  body.aft-dark-mode .hx-block .info-line b{color:#f1f5f9;}\n  body.aft-dark-mode .hx-block .ace-line{color:#cbd5e1;}\n  body.aft-dark-mode .hx-block .ace-line strong{color:#f1f5f9;}\n  body.aft-dark-mode .hx-end{color:#64748b;border-color:#334155;}\n  \/* ===== \u79fb\u52a8\u7aef ===== *\/\n  @media (max-width:760px){\n    .hx-grid{flex-direction:column;gap:14px;}\n    .hx-protocol{padding:16px;}\n    .hx-figure img{max-height:none;}\n  }\n<\/style>\n<div class=\"hx-article\">\n<p>  <!-- \u6848\u4f8b 10 \u4e0d\u542b\u89c6\u9891\u6309\u94ae (\u7528\u6237\u8981\u6c42\u5220\u9664) --><\/p>\n<h2>\u6848\u4f8b 10\uff1a\u9ad8\u4fdd\u771f\u8d85\u5206\u8fa8\u7ea7\u6837\u672c-\u7ec6\u80de\u5fae\u4e1d\u3001\u5fae\u7ba1\u6d3b\u7ec6\u80de STED \u6210\u50cf<\/h2>\n<p>  <!-- ===== 2 \u5217\u5e03\u5c40 ===== --><\/p>\n<div class=\"hx-grid\">\n<p>    <!-- ===== \u5de6\u680f\uff1a\u4e3b\u56fe + Protocol ===== --><\/p>\n<div class=\"hx-col\">\n<figure class=\"hx-figure\">\n        <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/08\/1703\/main.webp\" alt=\"\u7ec6\u80de\u5fae\u4e1d\u3001\u5fae\u7ba1\u6d3b\u7ec6\u80de STED \u6210\u50cf\"><br \/>\n      <\/figure>\n<div class=\"hx-protocol\">\n<p class=\"p-title\"><span class=\"ico\">\ud83e\uddea<\/span><span>Protocol for high-fidelity live Actin and Microtubule STED Sample<\/span><\/p>\n<ol>\n<li>\u8ffd\u6c42\u9ad8\u4fdd\u771f\u7684\u7ec6\u80de\u57f9\u517b\uff0c\u5e94\u7528 <span class=\"k\">\u7ec6\u80de\u9ad8\u6d3b\u529b\u57f9\u517b\u57fa AB \u6db2<\/span>\u5b9e\u73b0\u9ad8\u6d3b\u529b\u548c\u5065\u5eb7\u5ea6\u7684\u7ec6\u80de\u57f9\u517b\uff0c\u5e76\u5728\u79cd\u6210\u50cf\u5b50\u7ec6\u80de\u524d\u4f7f\u7528 <span class=\"k\">\u7ec6\u80de\u8d34\u58c1\u8f85\u52a9\u6db2<\/span>\u83b7\u5f97\u5145\u5206\u8d34\u58c1\u5ef6\u5c55\u7684\u7ec6\u80de\uff1b<\/li>\n<li>\u52a0\u5de5\u5de5\u4f5c\u6d53\u5ea6\u4e3a <code>1 \u03bcM<\/code> \u7684\u6d3b\u7ec6\u80de\u67d3\u6599\uff0c\u5916\u52a0 <code>10 \u03bcM<\/code> \u7684\u6807\u8bb0\u8f85\u52a9\u5242 <code>verapamil<\/code>\uff0c\u5728\u57f9\u517b\u7bb1\u5b75\u80b2\uff0c\u6807\u8bb0 1 h\uff0c\u672c\u6b21\u6807\u8bb0\u7684\u67d3\u6599\u662f\uff1a<code>abberior live610 actin<\/code> \u548c <code>abberior live550 tubulin<\/code>\uff1b<\/li>\n<li>\u5728\u6210\u50cf\u524d\uff0c\u66f4\u6362 <span class=\"k\">cytoshield \u6d3b\u7ec6\u80de\u6210\u50cf\u7f13\u51b2\u6db2<\/span>\uff0c\u4ee5\u83b7\u5f97\u6700\u4f4e\u7ec6\u80de\u6bd2\u6027\u3001\u6700\u957f\u65f6\u7a0b\u7684\u9ad8\u8d28\u91cf\u6d3b\u7ec6\u80de\u8d85\u5206\u8fa8\u6210\u50cf\u3002<\/li>\n<\/ol><\/div>\n<\/p><\/div>\n<p>    <!-- ===== \u53f3\u680f\uff1a\u89e3\u8bfb\u5757 ===== --><\/p>\n<div class=\"hx-col\">\n<div class=\"hx-block\">\n<h4>\u2b50 \u56fe\u50cf\u63cf\u8ff0\uff1a<\/h4>\n<p>\u56fe\u50cf\u9009\u53d6\u4e86\u7ec6\u80de\u5706\u6247\u5f62\u7684\u80de\u4f53\uff0c\u7ec6\u80de\u80de\u4f53\u6241\u5e73\u5730\u94fa\u5c55\u5728\u89c6\u91ce\u4e2d\uff0c\u5448\u73b0\u548c\u8c10\u7684\u5706\u5f62\u3002\u7ec6\u80de\u5185\u7684\u5fae\u4e1d\u5448\u73b0\u5e94\u529b\u7ea4\u7ef4\u548c\u7ec6\u4e1d\u7684\u8868\u578b\uff0c\u5fae\u7ba1\u8f90\u5c04\u72b6\u3002\u6574\u4e2a\u7ec6\u80de\u9aa8\u67b6\u5448\u73b0\u5c0f\u8303\u56f4\u7684\u6446\u52a8\uff0c\u652f\u6491\u7740\u7ec6\u80de\u5706\u5468\u5bf9\u79f0\u7684\u7ed3\u6784\u3002<br \/><b>\u5fae\u4e1d\uff1a<\/b>\u84dd\u8272\uff1b<b>\u5fae\u7ba1\uff1a<\/b>\u7d2b\u8272\u3002<\/p>\n<\/p><\/div>\n<div class=\"hx-block\">\n<h4>\u2b50 \u6807\u8bb0\u8981\u70b9\uff1a<\/h4>\n<ol>\n<li>\u68af\u5ea6\u6d4b\u8bd5\u67d3\u6599\u6d53\u5ea6\uff0c\u5728\u65e0\u660e\u663e\u7ec6\u80de\u6bd2\u6027\u60c5\u51b5\u4e0b\uff0c\u5c3d\u53ef\u80fd\u589e\u52a0\u67d3\u8272\u6d53\u5ea6\u548c\u65f6\u95f4\uff1b<\/li>\n<li>\u4f7f\u7528\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u67d3\u8272\uff0c\u907f\u514d\u8840\u6e05\u5438\u9644\u67d3\u6599\uff0c\u964d\u4f4e\u6709\u6548\u67d3\u8272\u6d53\u5ea6\uff1b<\/li>\n<li>\u4f7f\u7528\u6807\u8bb0\u8f85\u52a9\u5242\u589e\u52a0\u6807\u8bb0\u6548\u7387\uff0c\u6291\u5236\u79bb\u5b50\u6cf5\u5916\u6392\u7ec6\u80de\u5185\u7684\u67d3\u6599\uff1b<\/li>\n<li>\u67d3\u8272\u5b8c\u6bd5\u540e\u4f7f\u7528\u5b8c\u5168\u57f9\u517b\u57fa\u57f9\u517b&#8221;\u6e05\u6d17&#8221;\u67d3\u6599 6-12 \u5c0f\u65f6\uff0c\u4f7f\u7ec6\u80de\u5410\u51fa\u80de\u5185\u591a\u4f59\u67d3\u6599\uff1b<\/li>\n<li>\u7ec6\u80de\u72b6\u6001\u548c\u6d3b\u6027\u6781\u5176\u91cd\u8981\uff0c\u9700\u8981\u9075\u5faa\u7ec6\u80de\u57f9\u517b\u539f\u5219\u517b\u51fa\u9ad8\u6d3b\u529b\u7684\u7ec6\u80de\u3002<\/li>\n<\/ol><\/div>\n<div class=\"hx-block\">\n<h4>\u2728 \u6210\u50cf\u8981\u70b9\uff1a<\/h4>\n<ol>\n<li>\u5b8c\u6574\u6d3b\u7ec6\u80de\u6210\u50cf\u9700\u6ce8\u610f\uff0cSTED \u626b\u63cf\u65f6\u95f4\u53d6\u51b3\u4e8e\u632f\u5e45\uff0c\u4e14\u76f8\u5bf9\u65f6\u95f4\u8f83\u957f\u3002\u4e3a\u4fdd\u8bc1\u6574\u4e2a\u6d3b\u7ec6\u80de\u5728\u626b\u63cf\u65f6\u95f4\u5185\u6ca1\u6709\u5927\u5e45\u8fd0\u52a8\uff0c\u9700\u8981\u5728\u6241\u5e73\u5706\u6247\u5f62\u7684\u7ec6\u80de\u4e2d\uff0c\u9009\u62e9\u6574\u4f53\u4f53\u79ef\u76f8\u5bf9\u8f83\u5c0f\u7684\uff0c\u4ee5\u5c3d\u91cf\u51cf\u5c11\u626b\u63cf\u65f6\u95f4\u3002<\/li>\n<li>\u6d3b\u7ec6\u80de\u62cd\u6444\u8981\u70b9\uff0c\u9700\u8981\u68af\u5ea6\u6d4b\u8bd5\u66dd\u5149\u6761\u4ef6\uff0c\u5728 SNR \u8db3\u591f\u7684\u60c5\u51b5\u4e0b\uff0c\u5c3d\u53ef\u80fd\u964d\u4f4e\u6fc0\u5149\u548c\u66dd\u5149\uff0c\u4ee5\u5ef6\u957f\u6d3b\u7ec6\u80de\u62cd\u6444\u7684\u65f6\u95f4\u3002<\/li>\n<li>\u9501\u7126\/\u51c6\u7126\uff1a\u6052\u6e29\u5728\u663e\u5fae\u955c\u4e0a\u653e\u7f6e\u5341\u5206\u949f\uff0c\u6e29\u5ea6\u53d8\u5316\u548c\u673a\u68b0\u5f62\u53d8\u5168\u90e8\u8d8b\u4e8e\u7a33\u5b9a\uff0c\u66f4\u5229\u4e8e\u9501\u7126\u3002<\/li>\n<\/ol><\/div>\n<p>      <!-- ===== \u6807\u8bb0\u4fe1\u606f + \u6210\u50cf\u8bbe\u5907\uff08\u98de\u4e66 ace-line \u98ce\u683c\uff09 ===== --><\/p>\n<div class=\"hx-block\">\n<div data-lark-html-role=\"root\">\n<div class=\"ace-line\">\ud83c\udfa8<strong>\u6807\u8bb0\u4fe1\u606f\uff1a<\/strong><\/div>\n<div class=\"ace-line\">\u3000\u3000\u6d3b\u7ec6\u80de\u76f4\u6807\u67d3\u8272<\/div>\n<div class=\"ace-line\">\u3000\u3000\u67d3\u6599\uff1alive 610 actin\u3001live 550 tubulin<\/div>\n<div 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