{"id":1658,"date":"2026-07-28T05:59:01","date_gmt":"2026-07-28T05:59:01","guid":{"rendered":"https:\/\/www.hxicollege.com\/?p=1658"},"modified":"2026-07-28T16:37:43","modified_gmt":"2026-07-28T08:37:43","slug":"case-03-sample-3d-imaging-1658","status":"publish","type":"post","link":"https:\/\/www.hxicollege.com\/index.php\/2026\/07\/28\/case-03-sample-3d-imaging-1658\/","title":{"rendered":"\u6848\u4f8b 03\uff1a\u9ad8\u4fdd\u771f\u8d85\u5206\u8fa8\u7ea7\u6837\u672c-\u7ec6\u80de\u5fae\u7ba1\u7684 STORM 2D \u6210\u50cf"},"content":{"rendered":"<style>\n  \/* ===== \u6574\u7bc7\u5bb9\u5668 ===== *\/\n  .hx-article{\n    max-width:1080px;\n    margin:0 auto;\n    padding:0 28px 64px;\n    font-family:-apple-system,BlinkMacSystemFont,\"Segoe UI\",\"PingFang SC\",\"Hiragino Sans GB\",\"Microsoft YaHei\",sans-serif;\n    color:#1f2328;\n    line-height:1.85;\n    font-size:16px;\n  }\n  \/* ===== h2 \u6848\u4f8b\u6807\u9898\uff08h1 \u7531 post_title \u6e32\u67d3\uff09 ===== *\/\n  .hx-article 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display:block;width:100%;height:auto;max-height:640px;object-fit:contain;margin:0 auto;\n  }\n  \/* ===== Protocol \u5757\uff08Feishu \u622a\u56fe\u5de6\u4e0b\uff09 ===== *\/\n  .hx-protocol{\n    background:#f6f8ff;\n    border:1px solid #e3e8fb;\n    border-radius:12px;\n    padding:20px 22px 22px;\n    margin:0;\n  }\n  .hx-protocol .p-title{\n    display:flex;\n    align-items:flex-start;\n    gap:8px;\n    font-size:16px;\n    font-weight:800;\n    color:#2563eb;\n    line-height:1.45;\n    margin:0 0 14px;\n  }\n  .hx-protocol .p-title .ico{flex:0 0 auto;}\n  .hx-protocol ol{margin:0;padding:0;list-style:none;counter-reset:pc;}\n  .hx-protocol li{\n    counter-increment:pc;\n    position:relative;\n    padding-left:26px;\n    margin-bottom:13px;\n    color:#374151;\n    font-size:14px;\n    line-height:1.75;\n  }\n  .hx-protocol li::before{\n    content:counter(pc)\".\";\n    position:absolute;\n    left:0;\n    top:0;\n    color:#f97316;\n    font-weight:800;\n  }\n  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padding:2px 7px;\n    border-radius:4px;\n    color:#c026d3;\n    font-family:Menlo,Monaco,Consolas,monospace;\n    font-size:.92em;\n  }\n  small{color:#64748b;font-size:.85em;}\n  \/* ===== CelebNews \u591c\u95f4\u6a21\u5f0f\u9002\u914d ===== *\/\n  body.aft-dark-mode .hx-article,\n  body.aft-dark-mode .hx-article p{color:#e2e8f0;}\n  body.aft-dark-mode .hx-article h2{color:#f1f5f9;}\n  body.aft-dark-mode .hx-figure{background:#1e293b;box-shadow:0 1px 3px rgba(0,0,0,.3),0 0 0 1px #334155;}\n  body.aft-dark-mode .hx-protocol{background:#1e293b;border-color:#334155;}\n  body.aft-dark-mode .hx-protocol .p-title{color:#93c5fd;}\n  body.aft-dark-mode .hx-protocol li{color:#cbd5e1;}\n  body.aft-dark-mode .hx-protocol li::before{color:#fb923c;}\n  body.aft-dark-mode .hx-protocol .k{background:rgba(37,99,235,.25);color:#93c5fd;}\n  body.aft-dark-mode .hx-block h4{color:#f1f5f9;}\n  body.aft-dark-mode .hx-block p,\n  body.aft-dark-mode .hx-block ol,\n  body.aft-dark-mode .hx-block ol li{color:#cbd5e1;}\n  body.aft-dark-mode .hx-block .info-line{color:#cbd5e1;}\n  body.aft-dark-mode .hx-block .info-line b{color:#f1f5f9;}\n  body.aft-dark-mode .hx-end{color:#64748b;border-color:#334155;}\n  \/* ===== \u79fb\u52a8\u7aef\u56de\u9000 ===== *\/\n  @media (max-width:760px){\n    .hx-grid{flex-direction:column;gap:14px;}\n    .hx-protocol{padding:16px;}\n  }\n<\/style>\n<div class=\"hx-article\">\n<p>  <!-- ===== h2 \u6848\u4f8b\u6807\u9898\uff08h1 \u7531 post_title \u6e32\u67d3\uff09 ===== --><\/p>\n<h2>\u6848\u4f8b 03\uff1a\u9ad8\u4fdd\u771f\u8d85\u5206\u8fa8\u7ea7\u6837\u672c-\u7ec6\u80de\u5fae\u7ba1\u7684 STORM 2D \u6210\u50cf<\/h2>\n<p>  <!-- ===== 2 \u5217\u5e03\u5c40 ===== --><\/p>\n<div class=\"hx-grid\">\n<p>    <!-- ===== \u5de6\u680f\uff1a\u56fe + Protocol ===== --><\/p>\n<div class=\"hx-col\">\n<figure class=\"hx-figure\">\n        <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1658\/20260720-%E5%8D%8E%E5%A4%8F%E6%88%90%E5%83%8F%20HIAL%20%E7%9B%B4%E6%92%AD-01_05.webp\" alt=\"\u7ec6\u80de\u5fae\u7ba1 STORM 2D \u6210\u50cf\"><br \/>\n      <\/figure>\n<div class=\"hx-protocol\" style=\"margin-top:18px;\">\n<p class=\"p-title\"><span class=\"ico\">\ud83e\uddea<\/span><span>Protocol for high-fidelity Fixed STORM 2D Sample<\/span><\/p>\n<ol>\n<li>\u9ad8\u4fdd\u771f\u5fae\u7ba1\u56fa\u5b9a\uff0c\u4f7f\u7528<span class=\"k\">\u9ad8\u4fdd\u771f\u56fa\u5b9a\u6db2-\u5fae\u7ba1<\/span>37 \u2103\u56fa\u5b9a\u7ec6\u80de 10min\uff1b<\/li>\n<li>\u4f7f\u75282ml <span class=\"k\">\u8d85\u7eaf\u5236\u6837PBS<\/span>\u7b80\u77ed\u7684\u6e05\u6d17\u7ec6\u80de 3 \u6b21\uff1b<\/li>\n<li>\u538b\u5236\u80cc\u666f\u8367\u5149\uff0c\u4f7f\u75282ml\u914d\u7f6e\u597d\u7684 <span class=\"k\">\u81ea\u53d1\u8367\u5149\u6291\u5236\u5242<\/span>\uff0c\u5728\u5ba4\u6e29\u4e0b\u5b75\u80b2\u6837\u672c 7min\uff0c\u4f7f\u7528\u4fa7\u6446\u6447\u5e8a\u8f85\u52a9\u4ea4\u6362\u6db2\u4f53\uff1b<\/li>\n<li>\u4f7f\u75282ml <span class=\"k\">\u8d85\u7eaf\u5236\u6837PBS<\/span>\u7b80\u77ed\u7684\u6e05\u6d17\u7ec6\u80de 3 \u6b21\uff1b<\/li>\n<li>\u5c01\u95ed\u6297\u4f53\u975e\u7279\u5f02\u6027\u7ed3\u5408\u4f4d\u70b9\uff0c\u4f7f\u7528 50ul \u914d\u7f6e\u597d\u7684<span class=\"k\">\u5c01\u95ed\u6253\u5b54\u6db2<\/span>\uff0c\u540c\u65f6\u4f7f\u7528 <span class=\"k\">\u5236\u6837\u4f34\u4fa3-\u8d34\u58c1\u6837\u672c<\/span>\u8f85\u52a9\u6837\u672c\u7684\u5b75\u80b2\uff0c\u5ba4\u6e29\u4e0b\u5b75\u80b2\u7ec6\u80de 30 min\uff1b<\/li>\n<li>\u4f7f\u7528 <span class=\"k\">\u5236\u6837\u4f34\u4fa3-\u8d34\u58c1\u6837\u672c<\/span>\u8f85\u52a9\u4e00\u6297\u5b75\u80b2\uff0c\u4fdd\u6301\u6e7f\u6da6\u73af\u5883\uff0c\u5ba4\u6e29\u5b75\u80b2 1 \u5c0f\u65f6\u3002\u4e00\u6297\u9009\u62e9 beta-tubulin E7 mouse\uff1b<\/li>\n<li>\u4f7f\u7528 2ml <span class=\"k\">\u8d85\u7eaf\u5236\u6837PBS<\/span>\u6e05\u6d17\u4e00\u6297\uff0c5min \u4e00\u6b21\uff0c\u6e05\u6d173\u6b21\uff0c\u4f7f\u7528\u4fa7\u6446\u6447\u5e8a\u8f85\u52a9\u4ea4\u6362\u6db2\u4f53\uff1b<\/li>\n<li>\u62b5\u5fa1\u975e\u7279\u5f02\u6027\u8367\u5149\u67d3\u6599\u7ed3\u5408\uff0c\u4f7f\u752825ul <span class=\"k\">\u8367\u5149\u51c0\u5316\u589e\u5f3a\u6db2<\/span>\uff0c\u540c\u65f6\u4f7f\u7528 <span class=\"k\">\u5236\u6837\u4f34\u4fa3-\u8d34\u58c1\u6837\u672c<\/span>\u8f85\u52a9\u6837\u672c\u7684\u5b75\u80b2\uff0c\u5ba4\u6e29\u5b75\u80b2\u6837\u672c 30min\uff1b<\/li>\n<li>\u4f7f\u7528 <span class=\"k\">\u5236\u6837\u4f34\u4fa3-\u8d34\u58c1\u6837\u672c<\/span>\u8f85\u52a9\u4e8c\u6297\u5b75\u80b2\u3002\u4fdd\u6301\u6e7f\u6da6\u73af\u5883\uff0c\u5ba4\u6e29\u5b75\u80b2 1 \u5c0f\u65f6\u3002\u4e8c\u6297\u9009\u62e9 Alexa555 goat-anti-mouse IgG\u3002<\/li>\n<li>\u4f7f\u7528 2ml <span class=\"k\">\u8d85\u7eaf\u5236\u6837PBS<\/span>\u6e05\u6d17\u4e8c\u6297\uff0c5min \u4e00\u6b21\uff0c\u6e05\u6d173\u6b21\uff0c\u4f7f\u7528\u4fa7\u6446\u6447\u5e8a\u8f85\u52a9\u4ea4\u6362\u6db2\u4f53\uff1b<\/li>\n<li>\u4f7f\u7528 <span class=\"k\">\u8d85\u5206\u8fa8\u5149\u955c\u7ea7PFA-16%<\/span>\uff0c\u9884\u5236\u6210 4%\u6d53\u5ea6\uff0c\u53d6 500ul \u6d78\u6ca1\u6837\u54c1\u8fdb\u884c\u540e\u56fa\u5b9a\uff0c\u65f6\u95f410min\u3002<\/li>\n<li>\u5728\u6210\u50cf\u65f6\uff0c\u4f7f\u7528<span class=\"k\">Antifade\u6210\u50cf\u7f13\u51b2\u6db2<\/span>\uff0c\u8fdb\u884c STORM 2D\u6210\u50cf\u3002<\/li>\n<\/ol><\/div>\n<\/p><\/div>\n<p>    <!-- ===== \u53f3\u680f\uff1a\u2b50 \u89e3\u8bfb ===== --><\/p>\n<div class=\"hx-col\">\n<div class=\"hx-block\">\n<h4>\u2b50 \u56fe\u50cf\u63cf\u8ff0\uff1a<\/h4>\n<p>\u56fe\u50cf\u662f\u7ec6\u80de\u5c40\u90e8\u7684\u5fae\u7ba1\u7f51\u7edc\uff0cZ \u8f74\u4f4d\u4e8e\u7ec6\u80de\u5185\u90e8\u7684\u4e2d\u9ad8\u5904\uff0c\u4f4d\u7f6e\u662f\u9760\u8fd1\u7ec6\u80de\u6838\uff0c\u4e5f\u9760\u8fd1\u5fae\u7ba1\u7ec4\u7ec7\u4e2d\u5fc3\u7684\u5fae\u7ba1\u81f4\u5bc6\u533a\u57df\u3002\u5fae\u7ba1\u81f4\u5bc6\u4f46\u6839\u6839\u5206\u660e\uff0c\u4e1d\u72b6\u7ed3\u6784\u6e05\u6670\u4e14\u8fde\u7eed\uff0c\u5206\u5e03\u5728 3D \u8303\u56f4\u5185\u3002\u989c\u8272\u4ee3\u8868 Z \u8f74\u9ad8\u5ea6\u3002<\/p>\n<\/p><\/div>\n<div class=\"hx-block\">\n<h4>\u2b50 \u6807\u8bb0\u8981\u70b9\uff1a<\/h4>\n<ol>\n<li>\u57f9\u517b\u8d34\u58c1\u826f\u597d\u3001\u9ad8\u6d3b\u529b\u4e14\u7ec6\u80de\u4f53\u6241\u5e73\u7684\u7ec6\u80de\u3002<\/li>\n<li>\u5fae\u7ba1\u662f\u5bf9\u6e29\u5ea6\u6781\u5176\u654f\u611f\u7684\u7ec6\u80de\u5668\u3002\u4ece\u57f9\u517b\u7bb1\u53d6\u51fa\u5230\u56fa\u5b9a\u7684\u5168\u8fc7\u7a0b\uff0c\u5fc5\u987b\u4e25\u683c\u907f\u514d\u6e29\u5ea6\u6ce2\u52a8\u3002<\/li>\n<li>\u9009\u7528\u9002\u5408\u5fae\u7ba1\u7684\u9ad8\u4fdd\u771f\u56fa\u5b9a\u6db2\uff0c\u9632\u6b62\u5fae\u7ba1\u89e3\u805a\u3002<\/li>\n<li>\u4e3a\u4f7f\u80cc\u666f\u66f4\u5e72\u51c0\uff0c\u4f7f\u7528Quench\u8bd5\u5242\u6765\u6291\u5236\u81ea\u53d1\u8367\u5149\u3002<\/li>\n<li>\u4f7f\u7528\u6bd4\u5e38\u89c4\u63a8\u8350\u6d53\u5ea6\u66f4\u9ad8\u7684\u4e00\u6297\u6d53\u5ea6\u53bb\u8fdb\u884c\u8fc7\u9971\u548c\u6807\u8bb0\u3002<\/li>\n<\/ol><\/div>\n<div class=\"hx-block\">\n<h4>\u2728 \u6210\u50cf\u8981\u70b9\uff1a<\/h4>\n<ol>\n<li>\u7ec6\u80de\u5b58\u5728\u5f02\u8d28\u6027\uff0c\u7cbe\u5fc3\u6311\u9009\u89c6\u91ce\uff0c\u4ee5\u5c40\u90e8\u7ed3\u6784\u590d\u5408\u8981\u6c42\u4e3a\u4e3b\uff0c\u53ef\u4ee5\u4e0d\u5fc5\u4ee5\u7ec6\u80de\u6574\u4f53\u6241\u5e73\u5706\u6247\u5f62\u4e3a\u4e3b\uff0c\u9009\u62e9\u5fae\u7ba1\u660e\u4eae\u4e14\u8fde\u7eed\u7684\u533a\u57df\u3002<\/li>\n<li>\u8be5\u56fe\u50cf\u62cd\u6444\u4e86Z\u8f74\u8fde\u7eed7\u5c42\u4ee5\u4e0a\u7684\u56fe\u50cf\u6808\uff08Stacks\uff09\u3002\u5c42\u95f4\u8ddd\uff08Step size\uff09\u8bbe\u4e3a 0.05~0.1 \u03bcm\u3002<\/li>\n<li>3D \u5c42\u626b\u6570\u636e\uff0c\u975e\u5e38\u9002\u5408\u8fdb\u884c 3D 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