{"id":1654,"date":"2026-07-28T05:46:42","date_gmt":"2026-07-28T05:46:42","guid":{"rendered":"https:\/\/www.hxicollege.com\/?p=1654"},"modified":"2026-07-28T13:53:54","modified_gmt":"2026-07-28T05:53:54","slug":"case-04-sample-microtubules-2d-storm","status":"publish","type":"post","link":"https:\/\/www.hxicollege.com\/index.php\/2026\/07\/28\/case-04-sample-microtubules-2d-storm\/","title":{"rendered":"\u6848\u4f8b 04\uff1a\u9ad8\u4fdd\u771f\u8d85\u5206\u8fa8\u7ea7\u6837\u672c-\u7ec6\u80de\u5fae\u7ba1\u7684 2D STORM \u6210\u50cf"},"content":{"rendered":"<p><!-- \u6848\u4f8b 04\uff1a\u9ad8\u4fdd\u771f\u8d85\u5206\u8fa8\u7ea7\u6837\u672c-\u7ec6\u80de\u5fae\u7ba1\u7684 2D STORM \u6210\u50cf\n     \u5e03\u5c40:\u4e25\u683c\u4eff\u7167 \u6848\u4f8b 01 (post 1322)\n       1. \n\n<h2> \u6848\u4f8b\u6807\u9898 (h1 \u7531 post_title \u6e32\u67d3)\n       2. .hx-grid 2 \u5217\n          \u5de6\u680f: .hx-figure \u5355\u56fe\u5361 + .hx-protocol \u5757 (\u6d45\u84dd\u5e95 + \ud83e\uddea \u6807\u9898 + \u6a59\u8272\u7f16\u53f7)\n          \u53f3\u680f: .hx-block \u89e3\u8bfb\u94fe (\u56fe\u50cf\u63cf\u8ff0 \/ \u6807\u8bb0\u8981\u70b9 \/ \u6210\u50cf\u8981\u70b9 \/ \u6807\u8bb0\u4fe1\u606f \/ \u6210\u50cf\u6a21\u5f0f)\n       3. .hx-end \u6536\u5c3e\n     CSS: \u590d\u7528 case1 \u7c7b\u540d\u4e0e\u8272\u677f (hx-article \/ hx-grid \/ hx-col \/ hx-figure \/ hx-protocol \/ hx-block \/ hx-end)\n     \u89c4\u7ea6: \u9876\u90e8\u4e0d\u7559\u767d (padding-top=0 + \u9996 h2 padding \u538b\u534a)\n           ol+counter \u4e09\u4ef6\u5957 (list-style:none !important + list-style-type:none !important)\n           CelebNews \u591c\u95f4\u6a21\u5f0f (body.aft-dark-mode)\n           \u9876\u680f\u53ea\u7559\u6807\u9898\n     \u6587\u5b57: \u4e00\u5b57\u4e0d\u52a8 (\u6e90\u7a3f protocol \u6807\u9898 \"3D Confocal Sample\" \u4fdd\u7559) --><\/p>\n<style>\n  \/* ===== \u6574\u7bc7\u5bb9\u5668 ===== *\/\n  .hx-article{\n    max-width:1080px;\n    margin:0 auto;\n    padding:0 28px 64px;\n    font-family:-apple-system,BlinkMacSystemFont,\"Segoe UI\",\"PingFang SC\",\"Hiragino Sans GB\",\"Microsoft YaHei\",sans-serif;\n    color:#1f2328;\n    line-height:1.85;\n    font-size:16px;\n  }\n  \/* ===== h2 \u6848\u4f8b\u6807\u9898 (h1 \u7531 post_title \u6e32\u67d3, \u9996 h2 \u538b\u534a\u9632\u9876\u7559\u767d) ===== *\/\n  .hx-article > h2:first-of-type{\n    font-size:22px;\n    font-weight:800;\n    color:#0f172a;\n    margin:0 0 14px;\n    padding-top:8px;\n    line-height:1.4;\n  }\n  .hx-article h2{\n    font-size:22px;\n    font-weight:800;\n    color:#0f172a;\n    margin:0 0 28px;\n    line-height:1.4;\n  }\n  \/* ===== 2 \u5217\u5e03\u5c40 ===== *\/\n  .hx-grid{display:flex;gap:26px;align-items:flex-start;}\n  .hx-grid .hx-col{flex:1 1 50%;min-width:0;}\n  \/* ===== \u5355\u56fe\u5361 ===== *\/\n  .hx-figure{\n    margin:18px 0;\n    background:#fff;\n    border-radius:12px;\n    overflow:hidden;\n    box-shadow:0 1px 3px rgba(15,23,42,.05),0 0 0 1px #eef0f3;\n  }\n  .hx-figure img{\n    display:block;width:100%;height:auto;max-height:640px;object-fit:contain;margin:0 auto;\n  }\n  \/* ===== Protocol \u5757 (\u5de6\u4e0b, \u6d45\u84dd\u5e95 + \u84dd\u8272\u6807\u9898 + \u6a59\u8272\u7f16\u53f7) ===== *\/\n  .hx-protocol{\n    background:#f6f8ff;\n    border:1px solid #e3e8fb;\n    border-radius:12px;\n    padding:20px 22px 22px;\n    margin:0;\n  }\n  .hx-protocol .p-title{\n    display:flex;align-items:flex-start;gap:8px;\n    font-size:16px;font-weight:800;color:#2563eb;\n    line-height:1.45;margin:0 0 14px;\n  }\n  .hx-protocol .p-title .ico{flex:0 0 auto;}\n  .hx-protocol ol{margin:0;padding:0;list-style:none !important;list-style-type:none !important;counter-reset:pc;}\n  .hx-protocol > ol > li{list-style:none !important;list-style-type:none !important;}\n  .hx-protocol li{\n    counter-increment:pc;\n    position:relative;\n    padding-left:26px;\n    margin-bottom:13px;\n    color:#374151;\n    font-size:14px;\n    line-height:1.75;\n  }\n  .hx-protocol li::before{\n    content:counter(pc)\".\";\n    position:absolute;left:0;top:0;\n    color:#f97316;\n    font-weight:800;\n  }\n  .hx-protocol .k{\n    background:rgba(37,99,235,.12);\n    color:#2563eb;\n    padding:1px 6px;\n    border-radius:4px;\n    font-weight:600;\n    font-size:13px;\n  }\n  \/* ===== \u53f3\u680f\u89e3\u8bfb\u5757 ===== *\/\n  .hx-block{margin-bottom:22px;}\n  .hx-block h4{\n    font-size:15.5px;\n    font-weight:800;\n    color:#0f172a;\n    margin:0 0 8px;\n    display:flex;\n    align-items:center;\n    gap:6px;\n  }\n  .hx-block p{margin:0 0 6px;color:#374151;font-size:15px;}\n  .hx-block ol{margin:4px 0 0;padding-left:20px;color:#374151;font-size:14.5px;}\n  .hx-block ol li{margin-bottom:5px;}\n  .hx-block .info-line{margin:2px 0;color:#374151;font-size:14.5px;}\n  .hx-block .info-line b{color:#0f172a;font-weight:600;}\n  \/* ===== \u6536\u5c3e ===== *\/\n  .hx-end{\n    text-align:center;\n    color:#94a3b8;\n    font-size:13px;\n    margin:56px 0 0;\n    padding-top:22px;\n    border-top:1px dashed #e2e8f0;\n  }\n  \/* ===== inline ===== *\/\n  code{\n    background:#f1f5f9;\n    padding:2px 7px;\n    border-radius:4px;\n    color:#c026d3;\n    font-family:Menlo,Monaco,Consolas,monospace;\n    font-size:.92em;\n  }\n  small{color:#64748b;font-size:.85em;}\n  \/* ===== CelebNews \u591c\u95f4\u6a21\u5f0f\u9002\u914d ===== *\/\n  body.aft-dark-mode .hx-article,\n  body.aft-dark-mode .hx-article p{color:#e2e8f0;}\n  body.aft-dark-mode .hx-article h2{color:#f1f5f9;}\n  body.aft-dark-mode .hx-figure{background:#1e293b;box-shadow:0 1px 3px rgba(0,0,0,.3),0 0 0 1px #334155;}\n  body.aft-dark-mode .hx-protocol{background:#1e293b;border-color:#334155;}\n  body.aft-dark-mode .hx-protocol .p-title{color:#93c5fd;}\n  body.aft-dark-mode .hx-protocol li{color:#cbd5e1;}\n  body.aft-dark-mode .hx-protocol li::before{color:#fb923c;}\n  body.aft-dark-mode .hx-protocol .k{background:rgba(37,99,235,.25);color:#93c5fd;}\n  body.aft-dark-mode .hx-block h4{color:#f1f5f9;}\n  body.aft-dark-mode .hx-block p,\n  body.aft-dark-mode .hx-block ol,\n  body.aft-dark-mode .hx-block ol li{color:#cbd5e1;}\n  body.aft-dark-mode .hx-block .info-line{color:#cbd5e1;}\n  body.aft-dark-mode .hx-block .info-line b{color:#f1f5f9;}\n  body.aft-dark-mode .hx-end{color:#64748b;border-color:#334155;}\n  \/* ===== \u79fb\u52a8\u7aef\u56de\u9000 ===== *\/\n  @media (max-width:760px){\n    .hx-grid{flex-direction:column;gap:14px;}\n    .hx-protocol{padding:16px;}\n    .hx-figure img{max-height:none;}\n  }\n<\/style>\n<div class=\"hx-article\">\n<p>  <!-- ===== \u6848\u4f8b\u6807\u9898 (h2, h1 \u7531 post_title \u6e32\u67d3) ===== --><\/p>\n<h2>\u6848\u4f8b 04\uff1a\u9ad8\u4fdd\u771f\u8d85\u5206\u8fa8\u7ea7\u6837\u672c-\u7ec6\u80de\u5fae\u7ba1\u7684 2D STORM \u6210\u50cf<\/h2>\n<p>  <!-- ===== 2 \u5217\u5e03\u5c40 ===== --><\/p>\n<div class=\"hx-grid\">\n<p>    <!-- ===== \u5de6\u680f:\u56fe + Protocol ===== --><\/p>\n<div class=\"hx-col\">\n<figure class=\"hx-figure\">\n        <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1654\/webp_%E5%8E%9F%E5%9B%BE.webp\" alt=\"\u7ec6\u80de\u5fae\u7ba12D STORM\u6210\u50cf\"><br \/>\n      <\/figure>\n<div class=\"hx-protocol\">\n<p class=\"p-title\"><span class=\"ico\">\ud83e\uddea<\/span><span>Protocol for high-fidelity Fixed 3D Confocal Sample<\/span><\/p>\n<ol>\n<li>\u7ec6\u80de\u62bd\u63d0\uff0c\u4f7f\u7528<span class=\"k\">\u6837\u672c\u57fa\u8d28\u51c0\u6db2\u5316\u6db2<\/span>\uff0c37 \u2103\u62bd\u63d0\u7ec6\u80de 10\u79d2\uff1b<\/li>\n<li>\u9ad8\u4fdd\u771f\u5fae\u7ba1\u56fa\u5b9a\uff0c\u4f7f\u7528<span class=\"k\">\u9ad8\u4fdd\u771f\u56fa\u5b9a\u6db2-\u5fae\u7ba1<\/span>37 \u2103\u56fa\u5b9a\u7ec6\u80de 10min\uff1b<\/li>\n<li>\u4f7f\u75282ml <span class=\"k\">\u8d85\u7eaf\u5236\u6837PBS<\/span>\u7b80\u77ed\u7684\u6e05\u6d17\u7ec6\u80de 3 \u6b21\uff1b<\/li>\n<li>\u538b\u5236\u80cc\u666f\u8367\u5149\uff0c\u4f7f\u75282ml\u914d\u7f6e\u597d\u7684 <span class=\"k\">\u81ea\u53d1\u8367\u5149\u6291\u5236\u5242<\/span>\uff0c\u5728\u5ba4\u6e29\u4e0b\u5b75\u80b2\u6837\u672c 7min\uff0c\u4f7f\u7528\u4fa7\u6446\u6447\u5e8a\u8f85\u52a9\u4ea4\u6362\u6db2\u4f53\uff1b<\/li>\n<li>\u4f7f\u75282ml <span class=\"k\">\u8d85\u7eaf\u5236\u6837PBS<\/span>\u7b80\u77ed\u7684\u6e05\u6d17\u7ec6\u80de 3 \u6b21\uff1b<\/li>\n<li>\u5c01\u95ed\u6297\u4f53\u975e\u7279\u5f02\u6027\u7ed3\u5408\u4f4d\u70b9\uff0c\u4f7f\u7528 50ul \u914d\u7f6e\u597d\u7684<span class=\"k\">\u5c01\u95ed\u6253\u5b54\u6db2<\/span>\uff0c\u540c\u65f6\u4f7f\u7528 <span class=\"k\">\u5236\u6837\u4f34\u4fa3-\u8d34\u58c1\u6837\u672c<\/span>\u8f85\u52a9\u6837\u672c\u7684\u5b75\u80b2\uff0c\u5ba4\u6e29\u4e0b\u5b75\u80b2\u7ec6\u80de 30 min\uff1b<\/li>\n<li>\u4f7f\u7528 <span class=\"k\">\u5236\u6837\u4f34\u4fa3-\u8d34\u58c1\u6837\u672c<\/span>\u8f85\u52a9\u4e00\u6297\u5b75\u80b2\uff0c\u4fdd\u6301\u6e7f\u6da6\u73af\u5883\uff0c\u5ba4\u6e29\u5b75\u80b2 1 \u5c0f\u65f6\u3002\u4e00\u6297\u9009\u62e9 beta-tubulin E7 mouse\uff1b<\/li>\n<li>\u4f7f\u7528 2ml <span class=\"k\">\u8d85\u7eaf\u5236\u6837PBS<\/span>\u6e05\u6d17\u4e00\u6297\uff0c5min \u4e00\u6b21\uff0c\u6e05\u6d173\u6b21\uff0c\u4f7f\u7528\u4fa7\u6446\u6447\u5e8a\u8f85\u52a9\u4ea4\u6362\u6db2\u4f53\uff1b<\/li>\n<li>\u62b5\u5fa1\u975e\u7279\u5f02\u6027\u8367\u5149\u67d3\u6599\u7ed3\u5408\uff0c\u4f7f\u752825ul <span class=\"k\">\u8367\u5149\u51c0\u5316\u589e\u5f3a\u6db2<\/span>\uff0c\u540c\u65f6\u4f7f\u7528 <span class=\"k\">\u5236\u6837\u4f34\u4fa3-\u8d34\u58c1\u6837\u672c<\/span>\u8f85\u52a9\u6837\u672c\u7684\u5b75\u80b2\uff0c\u5ba4\u6e29\u5b75\u80b2\u6837\u672c 30min\uff1b<\/li>\n<li>\u4f7f\u7528 <span class=\"k\">\u5236\u6837\u4f34\u4fa3-\u8d34\u58c1\u6837\u672c<\/span>\u8f85\u52a9\u4e8c\u6297\u5b75\u80b2\u3002\u4fdd\u6301\u6e7f\u6da6\u73af\u5883\uff0c\u5ba4\u6e29\u5b75\u80b2 1 \u5c0f\u65f6\u3002\u4e8c\u6297\u9009\u62e9 Alexa555 goat-anti-mouse IgG\u3002<\/li>\n<li>\u4f7f\u7528 2ml <span class=\"k\">\u8d85\u7eaf\u5236\u6837PBS<\/span>\u6e05\u6d17\u4e8c\u6297\uff0c5min \u4e00\u6b21\uff0c\u6e05\u6d173\u6b21\uff0c\u4f7f\u7528\u4fa7\u6446\u6447\u5e8a\u8f85\u52a9\u4ea4\u6362\u6db2\u4f53\uff1b<\/li>\n<li>\u4f7f\u7528 <span class=\"k\">\u8d85\u5206\u8fa8\u5149\u955c\u7ea7PFA-16%<\/span>\uff0c\u9884\u5236\u6210 4%\u6d53\u5ea6\uff0c\u53d6 500ul \u6d78\u6ca1\u6837\u54c1\u8fdb\u884c\u540e\u56fa\u5b9a\uff0c\u65f6\u95f410min\u3002<\/li>\n<li>\u5728\u6210\u50cf\u65f6\uff0c\u4f7f\u7528<span class=\"k\">5MLM\u6210\u50cf\u7f13\u51b2\u6db2-STORM<\/span>\uff0c\u8fdb\u884c 3D STORM\u6210\u50cf\u3002<\/li>\n<\/ol><\/div>\n<\/p><\/div>\n<p>    <!-- ===== \u53f3\u680f:\u2b50 \u89e3\u8bfb ===== --><\/p>\n<div class=\"hx-col\">\n<div class=\"hx-block\">\n<h4>\u2b50 \u56fe\u50cf\u63cf\u8ff0\uff1a<\/h4>\n<p>\u56fe\u50cf\u662f\u7ec6\u80de\u5c40\u90e8\u5fae\u7ba1\u7f51\u683c\u76842D STORM \u6210\u50cf\uff0c\u4f4d\u7f6e\u662f\u5706\u6247\u5f62\u7684\u7ec6\u80de\u8fb9\u7f18\u6241\u5e73\u677f\u72b6\u4f2a\u8db3\u533a\u57df\uff0cZ \u8f74\u662f\u7ec6\u80de\u7684\u6700\u4f4e\u5904\u3002\u5fae\u7ba1\u7f51\u7a00\u758f\uff0c\u80cc\u666f\u5e72\u51c0\uff0c\u6807\u8bb0\u5bc6\u5ea6\u9ad8\uff0c\u91c7\u6837\u6570\u5145\u8db3\uff0c\u5206\u8fa8\u7387\u9ad8\uff0c\u53ef\u4ee5\u770b\u5230\u6e05\u6670\u7684\u5fae\u7ba1\u4e2d\u7a7a\u7ed3\u6784\u3002<\/p>\n<\/p><\/div>\n<div class=\"hx-block\">\n<h4>\u2b50 \u6807\u8bb0\u8981\u70b9\uff1a<\/h4>\n<ol>\n<li>\u57f9\u517b\u8d34\u58c1\u826f\u597d\u3001\u9ad8\u6d3b\u529b\u4e14\u7ec6\u80de\u4f53\u6241\u5e73\u7684\u7ec6\u80de\u3002<\/li>\n<li>\u5fae\u7ba1\u662f\u5bf9\u6e29\u5ea6\u6781\u5176\u654f\u611f\u7684\u7ec6\u80de\u5668\uff0c \u4ece\u57f9\u517b\u7bb1\u53d6\u51fa\u5230\u56fa\u5b9a\u7684\u5168\u8fc7\u7a0b\uff0c\u5fc5\u987b\u4e25\u683c\u907f\u514d\u6e29\u5ea6\u6ce2\u52a8\u3002<\/li>\n<li>\u9009\u7528\u9002\u5408\u5fae\u7ba1\u7684\u9ad8\u4fdd\u771f\u56fa\u5b9a\u6db2\uff0c\u9632\u6b62\u5fae\u7ba1\u89e3\u805a\u3002<\/li>\n<li>\u4e3a\u4f7f\u80cc\u666f\u66f4\u5e72\u51c0\uff0c\u4f7f\u7528Quench\u8bd5\u5242\u6765\u6291\u5236\u81ea\u53d1\u8367\u5149\u3002<\/li>\n<li>\u4f7f\u7528\u6bd4\u5e38\u89c4\u63a8\u8350\u6d53\u5ea6\u66f4\u9ad8\u7684\u4e00\u6297\u6d53\u5ea6\u53bb\u8fdb\u884c\u8fc7\u9971\u548c\u6807\u8bb0\u3002<\/li>\n<li>\u8ffd\u6c42\u6781\u81f4\u5e72\u51c0\u7684\u7ec6\u80de\u5185\u80cc\u666f\uff0c\u9700\u8981\u5bf9\u7ec6\u80de\u8d28\u8fdb\u884c\u62bd\u63d0\uff0c\u53bb\u9664\u7ec6\u80de\u8d28\u4e2d\u6e38\u79bb\u7684 tubulin \u86cb\u767d\uff0c\u5b83\u4eec\u4e5f\u53ef\u4ee5\u88ab\u6807\u8bb0\u4ece\u800c\u5f62\u6210\u80cc\u666f\u3002<\/li>\n<\/ol><\/div>\n<div class=\"hx-block\">\n<h4>\u2b50 \u6210\u50cf\u8981\u70b9\uff1a<\/h4>\n<ol>\n<li>\u7ec6\u80de\u5b58\u5728\u5f02\u8d28\u6027\uff0c\u7cbe\u5fc3\u6311\u9009\u89c6\u91ce\uff0c\u9009\u62e9\u7ec6\u80de\u6574\u4f53\u6241\u5e73\u5706\u5f62\uff0c\u5177\u6709\u677f\u72b6\u4f2a\u8db3\uff0c\u5fae\u7ba1\u7a00\u758f\uff0c\u7ec6\u80de\u5185\u80cc\u666f\u4f4e\uff0c\u5fae\u7ba1\u660e\u4eae\u4e14\u8fde\u7eed\u7684\u533a\u57df\uff0c\u4ee5\u5c40\u90e8\u7ed3\u6784\u590d\u5408\u8981\u6c42\u4e3a\u4e3b\u3002<\/li>\n<li>\u8c03\u8282\u4f7fSNR\uff08\u4fe1\u53f7\u80cc\u666f\u6bd4\uff09\u6700\u9ad8\u7684 TIRF \u89d2\u5ea6\uff0c\u6700\u9002\u914d\u5fae\u7ba1\u7684\u89d2\u5ea6\u4e0d\u4e00\u5b9a\u662f\u6700\u4e25\u683c\u7684 TIRF \u89d2\uff0c\u4f46\u662f\u5728\u4e25\u683c TIRF 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class=\"hx-block\">\n<h4>\u2b50 \u6807\u8bb0\u4fe1\u606f\uff1a<\/h4>\n<p class=\"info-line\"><b>IF<\/b><\/p>\n<p class=\"info-line\"><b>\u4e00\u6297\uff1a<\/b>beta tubulin E7<\/p>\n<p class=\"info-line\"><b>\u4e8c\u6297\uff1a<\/b>Alexa647<\/p>\n<\/p><\/div>\n<div class=\"hx-block\">\n<h4>\u2b50 \u6210\u50cf\u6a21\u5f0f\uff1a<\/h4>\n<p class=\"info-line\">\u7406\u8bba\u5206\u8fa8\u7387~20nm<\/p>\n<p class=\"info-line\">\u5b9e\u9645\u5206\u8fa8\u7387~50nm\uff08\u7eba\u4f53\u5c3a\u5bf8\u53e0\u52a0\uff09<\/p>\n<p class=\"info-line\">2D STORM mode<\/p>\n<p class=\"info-line\">ONI nanoimager<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/p><\/div>\n<p class=\"hx-end\">\u2014\u2014 \u6848\u4f8b 04 \u00b7 \u56fe\u6587\u5b9e\u9a8c\u68b3\u7406 \u2014\u2014<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u2b50 \u56fe\u50cf\u63cf\u8ff0\uff1a<br \/>\n\u56fe\u50cf\u662f\u7ec6\u80de\u5c40\u90e8\u5fae\u7ba1\u7f51\u683c\u76842D STORM 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