{"id":1638,"date":"2026-07-27T09:29:21","date_gmt":"2026-07-27T09:29:21","guid":{"rendered":"https:\/\/www.hxicollege.com\/?p=1638"},"modified":"2026-07-27T18:20:00","modified_gmt":"2026-07-27T10:20:00","slug":"alexa488-microtubules-report-1638","status":"publish","type":"post","link":"https:\/\/www.hxicollege.com\/index.php\/2026\/07\/27\/alexa488-microtubules-report-1638\/","title":{"rendered":"20240123 Alexa488\u5355\u8272\u5fae\u7ba1-\u9a6c\u5149\u9e4f\u8001\u5e08\u4ea4\u4ed8\u62a5\u544a"},"content":{"rendered":"<style>\n\/* ====== hx-article \u5bbd\u7248 1080px ====== *\/\n.hx-article {\n  max-width: 1080px;\n  margin: 0 auto;\n  padding: 0 28px 64px;\n  font-family: -apple-system,BlinkMacSystemFont,\"Segoe UI\",\"PingFang SC\",\"Hiragino Sans GB\",\"Microsoft YaHei\",sans-serif;\n  font-size: 16px;\n  line-height: 1.85;\n  color: #1f2328;\n  background: #ffffff;\n}\n.hx-article h1 { display: none; }\n.hx-article > h2:first-child {\n  font-size: 22px;\n  font-weight: 700;\n  color: #0f172a;\n  margin: 0 0 16px;\n  padding: 6px 12px;\n  border-left: 4px solid #2563eb;\n  background: linear-gradient(90deg,#f1f5f9,transparent 70%);\n  border-radius: 4px;\n}\n.hx-article h2 {\n  font-size: 22px;\n  font-weight: 700;\n  color: #0f172a;\n  margin: 36px 0 16px;\n  padding: 10px 14px;\n  border-left: 4px solid #2563eb;\n  background: linear-gradient(90deg,#f1f5f9,transparent 70%);\n  border-radius: 4px;\n}\n.hx-article h3 {\n  font-size: 18px;\n  font-weight: 700;\n  color: #0f172a;\n  margin: 24px 0 12px;\n  padding-left: 14px;\n  border-left: 4px solid #94a3b8;\n}\n.hx-article h4 {\n  font-size: 15px;\n  font-weight: 700;\n  color: #475569;\n  margin: 16px 0 6px;\n}\n.hx-article p { margin: 8px 0; color: #1f2328; }\n.hx-article em { color: #64748b; font-style: normal; }<\/p>\n<p>.hx-table {\n  width: 100%;\n  border-collapse: collapse;\n  margin: 12px 0 24px;\n  font-size: 14px;\n  box-shadow: 0 0 0 1px #e2e8f0;\n  border-radius: 8px;\n  overflow: hidden;\n}\n.hx-table th, .hx-table td {\n  padding: 10px 14px;\n  border-bottom: 1px solid #eef0f3;\n  text-align: left;\n  vertical-align: top;\n}\n.hx-table th {\n  background: #f1f5f9;\n  font-weight: 700;\n  color: #0f172a;\n}\n.hx-table tr:nth-child(even) td { background: #fafbfc; }<\/p>\n<p>.hx-figure {\n  margin: 16px 0;\n  padding: 8px;\n  background: #f8fafc;\n  border: 1px solid #eef0f3;\n  border-radius: 8px;\n  text-align: center;\n}\n.hx-figure img {\n  max-width: 100%;\n  height: auto;\n  border-radius: 4px;\n  display: block;\n  margin: 0 auto;\n}\n.hx-figure figcaption {\n  margin-top: 8px;\n  font-size: 13px;\n  color: #64748b;\n}<\/p>\n<p>.hx-grid-3 {\n  display: grid;\n  grid-template-columns: repeat(3, 1fr);\n  gap: 12px;\n  margin: 16px 0 24px;\n}\n.hx-grid-3 .hx-figure { margin: 0; }<\/p>\n<p>.hx-callout {\n  padding: 14px 18px;\n  margin: 18px 0;\n  border-left: 4px solid #2563eb;\n  background: #eff6ff;\n  border-radius: 4px;\n  color: #1e3a8a;\n  font-size: 15px;\n}\n.hx-callout b { color: inherit; font-weight: 700; }<\/p>\n<p>@media (max-width:780px) {\n  .hx-article { padding: 24px 16px; }\n  .hx-grid-3 { grid-template-columns: 1fr; }\n}<\/p>\n<p>\/* ====== CelebNews \u591c\u95f4\u6a21\u5f0f\u9002\u914d ====== *\/\nbody.aft-dark-mode .hx-article { background:#0f172a; color:#e2e8f0; }\nbody.aft-dark-mode .hx-article p { color:#e2e8f0; }\nbody.aft-dark-mode .hx-article h2 { color:#f1f5f9; background: linear-gradient(90deg,#1e293b,transparent 70%); }\nbody.aft-dark-mode .hx-article h3 { color:#f1f5f9; border-left-color:#64748b; }\nbody.aft-dark-mode .hx-article h4 { color:#cbd5e1; }\nbody.aft-dark-mode .hx-table { background:#1e293b; box-shadow:0 0 0 1px #334155; }\nbody.aft-dark-mode .hx-table th { background:#0f172a; color:#f1f5f9; border-bottom-color:#334155; }\nbody.aft-dark-mode .hx-table td { background:#1e293b; color:#e2e8f0; border-bottom-color:#334155; }\nbody.aft-dark-mode .hx-table tr:nth-child(even) td { background:#172033; }\nbody.aft-dark-mode .hx-figure { background:#0f172a; border-color:#334155; }\nbody.aft-dark-mode .hx-figure figcaption { color:#94a3b8; }\n<\/style>\n<div class=\"hx-article\">\n<h2>\u4e00\u3001\u5236\u6837\u539f\u6599\u548c\u73af\u5883\u6761\u4ef6\u7559\u6863<\/h2>\n<h3>1.1 \u539f\u6599\u7167\u7247<\/h3>\n<h3>1.2 \u73af\u5883\u6761\u4ef6\u7559\u6863\uff1a<\/h3>\n<table class=\"hx-table\">\n<thead>\n<tr>\n<th>\u9879\u76ee<\/th>\n<th>\u6761\u4ef6 \/ \u6570\u503c<\/th>\n<th>\u5f02\u5e38\u60c5\u51b5\u5907\u6ce8<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>\u73af\u5883\u6e29\u5ea6<\/td>\n<td>25 \u2103<\/td>\n<td>\u2014<\/td>\n<\/tr>\n<tr>\n<td>\u73af\u5883\u6e7f\u5ea6<\/td>\n<td>30 %<\/td>\n<td>\u2014<\/td>\n<\/tr>\n<tr>\n<td>\u67d3\u8272\u65f6\u5de5\u4f5c\u6db2\u6e29\u5ea6<\/td>\n<td>25 \u2103<\/td>\n<td>\u504f\u4f4e\uff0c\u7ea6\u7b49\u4e8e\u5ba4\u5185\u6e29\u5ea6<\/td>\n<\/tr>\n<tr>\n<td>\u67d3\u6599\u50a8\u5b58\u65b9\u5f0f<\/td>\n<td>4 \u2103<\/td>\n<td>\u2014<\/td>\n<\/tr>\n<tr>\n<td>\u67d3\u6599\u50a8\u6db2\u6d53\u5ea6<\/td>\n<td>1 mM<\/td>\n<td>\u2014<\/td>\n<\/tr>\n<tr>\n<td>\u6297\u4f53\u50a8\u5b58\u65b9\u5f0f<\/td>\n<td>\u221220 \u2103<\/td>\n<td>\u2014<\/td>\n<\/tr>\n<tr>\n<td>\u6297\u4f53\u50a8\u6db2\u6d53\u5ea6<\/td>\n<td>1 mg\/ml<\/td>\n<td>\u2014<\/td>\n<\/tr>\n<tr>\n<td>\u6837\u672c\u7c7b\u578b\u548c\u540d\u8bcd<\/td>\n<td>BSC1 \u8f7d\u73bb\u7247<\/td>\n<td>\u2014<\/td>\n<\/tr>\n<tr>\n<td>\u6837\u672c\u524d\u5904\u7406\u64cd\u4f5c<\/td>\n<td>8% PFA + 1.5% GA \u56fa\u5b9a<\/td>\n<td>\u2014<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>\u4e8c\u3001\u6210\u50cf\u53c2\u6570<\/h2>\n<figure class=\"hx-figure\"><img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1638\/Untitled.webp\" alt=\"Olympus FV3000 \u6e32\u67d3\u53c2\u6570\u622a\u56fe\" \/><figcaption>\u6ce2\u957f\u3001Intensity\u3001\u7269\u955c\u653e\u5927\u500d\u6570\u3001pinhole size \u7b49\u53c2\u6570\u4f53\u73b0\u5728\u622a\u56fe\u4e2d \u2014\u2014 FV3000 \u6e32\u67d3\u65b9\u5f0f\uff1araw data<\/figcaption><\/figure>\n<h3>2.1 \u5149\u6e90 \/ \u5149\u8def \/ \u68c0\u6d4b\u5668\u914d\u7f6e<\/h3>\n<table class=\"hx-table\">\n<thead>\n<tr>\n<th>\u5149\u6e90<\/th>\n<th>\u6570\u503c \/ \u578b\u53f7<\/th>\n<th>\u5149\u8def<\/th>\n<th>\u6570\u503c<\/th>\n<th>\u68c0\u6d4b\u5668<\/th>\n<th>\u6570\u503c<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>\u6fc0\u5149\u5668\u603b\u529f\u7387<\/td>\n<td>405 \/ 50 mW \u00b7 488 \/ 20 mW \u00b7 561 \/ 20 mW \u00b7 640 \/ 40 mW<\/td>\n<td>\u6837\u54c1\u6eb6\u5242\u6298\u5c04\u7387<\/td>\n<td>1.0003<\/td>\n<td>\u76f8\u673a\u578b\u53f7<\/td>\n<td>IX3-LHLEDC<\/td>\n<\/tr>\n<tr>\n<td>\u6fc0\u5149\u51fa\u7269\u955c\u529f\u7387<\/td>\n<td>405 \/ 2 mW \u00b7 488 \/ 1.2 mW \u00b7 561 \/ 1.1 mW \u00b7 647 \/ 0.7 mW<\/td>\n<td>\u955c\u6cb9\u6298\u5c04\u7387<\/td>\n<td>1.5180<\/td>\n<td>HV<\/td>\n<td>500 V<\/td>\n<\/tr>\n<tr>\n<td>\u8bbe\u5907\u6210\u50cf\u6a21\u5f0f<\/td>\n<td>confocal<\/td>\n<td>\u6210\u50cf\u73bb\u7483\u578b\u53f7\u548c\u6298\u5c04\u7387<\/td>\n<td>\u8010\u601d\u76bf\uff0c\u9ad8\u5e73\u73bb\u7483\uff0c0.15 mm<\/td>\n<td>EMGain<\/td>\n<td>1.000<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>\u4e09\u3001\u5b9e\u9a8c\u7ed3\u679c<\/h2>\n<h3>3.1 \u6837\u672c\u4fe1\u606f<\/h3>\n<table class=\"hx-table\">\n<thead>\n<tr>\n<th colspan=\"2\">\u6837\u672c S1 \u2014\u2014 \u5fae\u7ba1\u6807\u8bb0<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<th>\u6837\u54c1\u4fe1\u606f<\/th>\n<td>\u6807\u8bb0\u5185\u5bb9\uff1a\u5fae\u7ba1 \u00b7 \u4e00\u6297 E7 mouse \u00b7 \u4e8c\u6297 Alexa Fluor 488 \u6807\u8bb0\u5c71\u7f8a\u6297\u5c0f\u9f20 IgG<\/td>\n<\/tr>\n<tr>\n<th>\u56fa\u5b9a\u6761\u4ef6<\/th>\n<td>8% PFA + 1.5% GA<\/td>\n<\/tr>\n<tr>\n<th>\u5c01\u95ed\u65f6\u95f4<\/th>\n<td>30 min<\/td>\n<\/tr>\n<tr>\n<th>\u4e00\u6297\u540d\u79f0<\/th>\n<td>E7 mouse<\/td>\n<\/tr>\n<tr>\n<th>\u4e00\u6297\u65f6\u95f4<\/th>\n<td>4 \u2103 \u4e24\u5929\u5b75\u80b2<\/td>\n<\/tr>\n<tr>\n<th>\u4e8c\u6297\u540d\u79f0<\/th>\n<td>Alexa Fluor 488 \u6807\u8bb0\u5c71\u7f8a\u6297\u5c0f\u9f20 IgG<\/td>\n<\/tr>\n<tr>\n<th>\u4e8c\u6297\u65f6\u95f4<\/th>\n<td>4 \u2103 \u4e00\u5929\u5b75\u80b2<\/td>\n<\/tr>\n<tr>\n<th>\u67d3\u6599\u540d\u79f0<\/th>\n<td>\/<\/td>\n<\/tr>\n<tr>\n<th>\u67d3\u6599\u65f6\u95f4<\/th>\n<td>\/<\/td>\n<\/tr>\n<tr>\n<th>\u662f\u5426\u7ecf\u8fc7\u79bb\u5fc3\u53bb\u9664\u805a\u6c89<\/th>\n<td>\u5426<\/td>\n<\/tr>\n<tr>\n<th>\u662f\u5426\u4e3a\u542b\u6c34\u6837\u672c<\/th>\n<td>\u5426<\/td>\n<\/tr>\n<tr>\n<th>\u662f\u5426\u56fa\u5b9a\u6216\u8005\u5c01\u7247<\/th>\n<td>\u662f<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3>3.2 10\u00d7 \u6982\u89c8\uff08488 \u901a\u9053\uff09<\/h3>\n<figure class=\"hx-figure\"><img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1638\/20240123-BP-10X-488.webp\" alt=\"10\u00d7 \u6982\u89c8\" \/><figcaption>10\u00d7 \u7269\u955c \u00b7 Alexa 488 \u901a\u9053 \u00b7 \u5fae\u7ba1\u6574\u4f53\u5206\u5e03<\/figcaption><\/figure>\n<h3>3.3 60\u00d7 \u89c6\u91ce\u5bf9\u6bd4 \u2014\u2014 488 \u901a\u9053<\/h3>\n<div class=\"hx-grid-3\">\n<figure class=\"hx-figure\"><img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1638\/20240123-BP-C01-60X-488.webp\" alt=\"C01 \u00b7 488\" \/><figcaption>C01 \u00b7 488<\/figcaption><\/figure>\n<figure class=\"hx-figure\"><img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1638\/20240123-BP-C02-60X-488.webp\" alt=\"C02 \u00b7 488\" \/><figcaption>C02 \u00b7 488<\/figcaption><\/figure>\n<figure class=\"hx-figure\"><img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1638\/20240123-BP-C03-60X-488.webp\" alt=\"C03 \u00b7 488\" \/><figcaption>C03 \u00b7 488<\/figcaption><\/figure>\n<\/div>\n<h3>3.4 60\u00d7 \u89c6\u91ce\u5bf9\u6bd4 \u2014\u2014 BF \u901a\u9053<\/h3>\n<div class=\"hx-grid-3\">\n<figure class=\"hx-figure\"><img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1638\/20240123-BP-C01-60X-BF.webp\" alt=\"C01 \u00b7 BF\" \/><figcaption>C01 \u00b7 BF<\/figcaption><\/figure>\n<figure class=\"hx-figure\"><img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1638\/20240123-BP-C02-60X-BF.webp\" alt=\"C02 \u00b7 BF\" \/><figcaption>C02 \u00b7 BF<\/figcaption><\/figure>\n<figure class=\"hx-figure\"><img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1638\/20240123-BP-C03-60X-BF.webp\" alt=\"C03 \u00b7 BF\" \/><figcaption>C03 \u00b7 BF<\/figcaption><\/figure>\n<\/div>\n<h3>3.5 \u539f\u6587\u4ef6<\/h3>\n<table class=\"hx-table\">\n<tbody>\n<tr>\n<th>10\u00d7 \u00b7 488<\/th>\n<td>20240123-BP-10X-488.oir<\/td>\n<\/tr>\n<tr>\n<th>C01 \u00b7 488<\/th>\n<td>20240123-BP-C01-60X-488.oir<\/td>\n<\/tr>\n<tr>\n<th>C01 \u00b7 BF<\/th>\n<td>20240123-BP-C01-60X-BF.oir<\/td>\n<\/tr>\n<tr>\n<th>C02 \u00b7 488<\/th>\n<td>20240123-BP-C02-60X-488.oir<\/td>\n<\/tr>\n<tr>\n<th>C02 \u00b7 BF<\/th>\n<td>20240123-BP-C02-60X-BF.oir<\/td>\n<\/tr>\n<tr>\n<th>C03 \u00b7 488<\/th>\n<td>20240123-BP-C03-60X-488.oir<\/td>\n<\/tr>\n<tr>\n<th>C03 \u00b7 BF<\/th>\n<td>20240123-BP-C03-60X-BF.oir<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u4e00\u3001\u3010\u5236\u6837\u539f\u6599\u548c\u73af\u5883\u6761\u4ef6\u7559\u6863\u3011<br \/>\n1.1 \u539f\u6599\u7167\u7247<\/p>\n<p>1.2 \u73af\u5883\u6761\u4ef6\u7559\u6863\uff1a<\/p>\n<p>\u8bf4\u660e<br \/>\n\u5f02\u5e38\u60c5\u51b5\u5907\u6ce8<br \/>\n\u73af\u5883\u6e29\u5ea6<br \/>\n25\u2103<\/p>\n<p>\u73af\u5883\u6e7f\u5ea6<br \/>\n30%<\/p>\n<p>\u67d3\u8272\u65f6\u5de5\u4f5c\u6db2\u6e29\u5ea6<br \/>\n25 \u2103<br \/>\n\u504f\u4f4e\uff0c\u7ea6\u7b49\u4e8e\u5ba4\u5185\u6e29\u5ea6<br \/>\n\u67d3\u6599\u50a8\u5b58\u65b9\u5f0f<br \/>\n 4\u2103<\/p>\n<p>\u67d3\u6599\u50a8\u6db2\u6d53\u5ea6<br \/>\n1mM<\/p>\n<p>\u6297\u4f53\u50a8\u5b58\u65b9\u5f0f<br \/>\n-20\u2103<\/p>\n<p>\u6297\u4f53\u50a8\u6db2\u6d53\u5ea6<br \/>\n1mg\/ml<\/p>\n<p>\u6837\u672c\u7c7b\u578b\u548c\u540d\u8bcd<br \/>\nBSC1\u8f7d\u73bb\u7247<\/p>\n<p>\u6837\u672c\u524d\u5904\u7406\u64cd\u4f5c<br \/>\n8%PFA+1.5%GA\u56fa\u5b9a<\/p>\n","protected":false},"author":1,"featured_media":1652,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[52,55],"tags":[48],"class_list":["post-1638","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-customer-case","category-select-case","tag-main"],"_links":{"self":[{"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/posts\/1638","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/comments?post=1638"}],"version-history":[{"count":3,"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/posts\/1638\/revisions"}],"predecessor-version":[{"id":1653,"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/posts\/1638\/revisions\/1653"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/media\/1652"}],"wp:attachment":[{"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/media?parent=1638"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/categories?post=1638"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/tags?post=1638"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}