{"id":1617,"date":"2026-07-26T15:18:28","date_gmt":"2026-07-26T15:18:28","guid":{"rendered":"https:\/\/www.hxicollege.com\/?p=1617"},"modified":"2026-07-27T17:57:43","modified_gmt":"2026-07-27T09:57:43","slug":"case-02-sample-microtubules-1617","status":"publish","type":"post","link":"https:\/\/www.hxicollege.com\/index.php\/2026\/07\/26\/case-02-sample-microtubules-1617\/","title":{"rendered":"\u6848\u4f8b 02\uff1a\u9ad8\u4fdd\u771f\u8d85\u5206\u8fa8\u7ea7\u6837\u672c-\u7ec6\u80de\u5fae\u7ba1\u6d3b\u7ec6\u80de\u5171\u805a\u7126\u6210\u50cf"},"content":{"rendered":"<p><!-- \u6848\u4f8b 02\uff1a\u9ad8\u4fdd\u771f\u8d85\u5206\u8fa8\u7ea7\u6837\u672c-\u7ec6\u80de\u5fae\u7ba1\u6d3b\u7ec6\u80de\u5171\u805a\u7126\u6210\u50cf\n     \u5e03\u5c40 v8: \u4e25\u683c\u4eff\u7167 \u6848\u4f8b 01 (post 1322) \u5e03\u5c40\u7ed3\u6784\n       1. \ud83d\udcfa \u89c6\u9891\u94fe\u63a5\u5c45\u4e2d\u6309\u94ae\n       2. \n\n<h2> \u6848\u4f8b\u6807\u9898 (\u4e0e post_title \u540c\u884c, 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--><\/p>\n<style>\n  \/* ===== \u6574\u7bc7\u5bb9\u5668 ===== *\/\n  .hx-article{\n    max-width:1080px;\n    margin:0 auto;\n    padding:40px 28px 64px;\n    font-family:-apple-system,BlinkMacSystemFont,\"Segoe UI\",\"PingFang SC\",\"Hiragino Sans GB\",\"Microsoft YaHei\",sans-serif;\n    color:#1f2328;\n    line-height:1.85;\n    font-size:16px;\n  }\n  \/* ===== h2 \u6848\u4f8b\u6807\u9898 (h1 \u7531 post_title \u6e32\u67d3) ===== *\/\n  .hx-article h2{\n    font-size:22px;\n    font-weight:800;\n    color:#0f172a;\n    margin:0 0 28px;\n    line-height:1.4;\n  }\n  \/* ===== \u89c6\u9891\u6309\u94ae ===== *\/\n  \/* ===== 2 \u5217\u5e03\u5c40 ===== *\/\n  .hx-grid{display:flex;gap:26px;align-items:flex-start;}\n  .hx-grid .hx-col{flex:1 1 50%;min-width:0;}\n  \/* ===== \u5355\u56fe\u5361 ===== *\/\n  .hx-figure{\n    margin:18px 0;\n    position:relative;\n    background:#000;\n    border-radius:12px;\n    overflow:hidden;\n    box-shadow:0 1px 3px rgba(15,23,42,.05),0 0 0 1px #eef0f3;\n  }\n  .hx-figure img{\n    display:block;width:100%;height:auto;max-height:640px;object-fit:contain;margin:0 auto;\n  }\n  \/* ===== \u25b6 \u89d2\u6807 (\u52a8\u56fe\u4e2d\u592e, case1 \u6ca1\u6709\u52a8\u56fe, \u8fd9\u91cc\u65b0\u589e) ===== *\/\n  \/* ===== Protocol \u5757 (\u5de6\u680f\u5e95\u90e8, \u4e0e case1 \u4e00\u81f4) ===== *\/\n  .hx-protocol{\n    background:#f6f8ff;\n    border:1px solid #e3e8fb;\n    border-radius:12px;\n    padding:20px 22px 22px;\n    margin:0;\n  }\n  .hx-protocol .p-title{\n    display:flex;align-items:flex-start;gap:8px;\n    font-size:16px;font-weight:800;color:#2563eb;\n    line-height:1.45;margin:0 0 14px;\n  }\n  .hx-protocol .p-title .ico{flex:0 0 auto;}\n  .hx-protocol ol{margin:0;padding:0;list-style:none !important;list-style-type:none !important;counter-reset:pc;}\n  .hx-protocol > ol > li{list-style:none !important;list-style-type:none !important;}\n  .hx-protocol li{\n    counter-increment:pc;\n    position:relative;\n    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.info-line{color:#cbd5e1;}\n  body.aft-dark-mode .hx-block .info-line b{color:#f1f5f9;}\n  body.aft-dark-mode .hx-block .ace-line{color:#cbd5e1;}\n  body.aft-dark-mode .hx-block .ace-line strong{color:#f1f5f9;}\n  body.aft-dark-mode .hx-end{color:#64748b;border-color:#334155;}\n  \/* ===== \u79fb\u52a8\u7aef\u56de\u9000 (\u4e0e case1 \u4e00\u81f4) ===== *\/\n  @media (max-width:760px){\n    .hx-grid{flex-direction:column;gap:14px;}\n    .hx-protocol{padding:16px;}\n    .hx-figure img{max-height:none;}\n  }\n<\/style>\n<div class=\"hx-article\">\n<p>  <!-- ===== \u6848\u4f8b\u6807\u9898 (h2, \u4e0e case1 \u4e00\u81f4) ===== --><\/p>\n<h2>\u6848\u4f8b 02\uff1a\u9ad8\u4fdd\u771f\u8d85\u5206\u8fa8\u7ea7\u6837\u672c-\u7ec6\u80de\u5fae\u7ba1\u6d3b\u7ec6\u80de\u5171\u805a\u7126\u6210\u50cf<\/h2>\n<p>  <!-- ===== 2 \u5217\u5e03\u5c40 ===== --><\/p>\n<div class=\"hx-grid\">\n<p>    <!-- ===== \u5de6\u680f: \u52a8\u56fe + Protocol ===== --><\/p>\n<div class=\"hx-col\">\n<figure class=\"hx-figure\">\n        <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1617\/motion.webp\" alt=\"\u5fae\u7ba1\u6d3b\u7ec6\u80de\u52a8\u6001\u6210\u50cf\"><br \/>\n        <\/figure>\n<div class=\"hx-protocol\">\n<p class=\"p-title\"><span class=\"ico\">\ud83e\uddea<\/span><span>Protocol for high-fidelity live Microtubule STED Sample<\/span><\/p>\n<ol>\n<li>\u9ad8\u4fdd\u771f\u7684\u7ec6\u80de\u57f9\u517b\uff0c\u5e94\u7528 <span class=\"k\">\u7ec6\u80de\u9ad8\u6d3b\u529b\u57f9\u517b\u57fa AB \u6db2<\/span>\u5b9e\u73b0\u9ad8\u6d3b\u529b\u548c\u5065\u5eb7\u5ea6\u7684\u7ec6\u80de\u57f9\u517b\uff0c\u5e76\u5728\u79cd\u6210\u8c61\u5b50\u7ec6\u80de\u524d\u4f7f\u7528 <span class=\"k\">\u7ec6\u80de\u8d34\u58c1\u8f85\u52a9\u6db2<\/span>\u83b7\u5f97\u5145\u5206\u8d34\u58c1\u5ef6\u5c55\u7684\u7ec6\u80de\uff1b<\/li>\n<li>\u52a0\u5165\u5de5\u4f5c\u6d53\u5ea6\u4e3a <code>1 \u03bcM<\/code> \u7684\u6d3b\u7ec6\u80de\u67d3\u6599\uff0c\u5916\u52a0 <code>10 \u03bcM<\/code> \u7684\u6807\u8bb0\u8f85\u52a9\u5242 <code>verapamil<\/code>\uff0c\u5728\u57f9\u517b\u7bb1\u5b75\u80b2\uff0c\u6807\u8bb0 1 h\uff0c\u672c\u6b21\u6807\u8bb0\u7684\u67d3\u6599\u662f <code>abberior live550 tubulin<\/code>\uff1b<\/li>\n<li>\u5728\u6210\u50cf\u524d\uff0c\u66f4\u6362 <span class=\"k\">cytoshield \u6d3b\u7ec6\u80de\u6210\u50cf\u7f13\u51b2\u6db2<\/span>\uff0c\u4ee5\u83b7\u5f97\u6700\u4f4e\u7ec6\u80de\u6bd2\u6027\u3001\u6700\u957f\u65f6\u7a0b\u7684\u9ad8\u8d28\u91cf\u6d3b\u7ec6\u80de\u8d85\u5206\u8fa8\u6210\u50cf\u3002<\/li>\n<\/ol><\/div>\n<\/p><\/div>\n<p>    <!-- ===== \u53f3\u680f: \u89e3\u8bfb\u5757 (\u4e0e case1 \u7ed3\u6784\u5bf9\u9f50: \u56fe\u50cf\u63cf\u8ff0 \/ \u6807\u8bb0\u8981\u70b9 \/ \u6210\u50cf\u8981\u70b9 \/ \u6807\u8bb0\u4fe1\u606f \/ \u6210\u50cf\u8bbe\u5907) ===== --><\/p>\n<div class=\"hx-col\">\n<div class=\"hx-block\">\n<h4>\u2b50 \u56fe\u50cf\u63cf\u8ff0\uff1a<\/h4>\n<p>\u6d3b\u7ec6\u80de\u5171\u805a\u7126\u6210\u50cf\uff0c\u6574\u4e2a\u7ec6\u80de\u5448\u73b0\u51fa\u5706\u6247\u5f62\uff0c\u6241\u5e73\u5730\u94fa\u5c55\u5728\u89c6\u91ce\u4e2d\uff0c\u7ec6\u80de\u95f4\u7684\u5fae\u7ba1\u5448\u8f90\u5c04\u72b6\u6392\u5217\uff0c\u8fde\u7eed\u3001\u5747\u5300\u3001\u660e\u4eae\u3002\u8fd9\u662f\u4e00\u5f20 2D \u5171\u805a\u7126\u6210\u50cf\u65f6\u95f4\u5e8f\u5217\uff0c\u81ea\u5b9a\u4e49\u989c\u8272\u533a\u5206\u4eae\u5ea6\uff0c<strong>\u7eff\u8272\u4ee3\u8868\u4fe1\u53f7\u8f83\u9ad8\u7684\u533a\u57df\uff0c\u7d2b\u8272\u4ee3\u8868\u4fe1\u53f7\u8f83\u4f4e\u7684\u533a\u57df<\/strong>\u3002<\/p>\n<\/p><\/div>\n<div class=\"hx-block\">\n<h4>\u2b50 \u6807\u8bb0\u8981\u70b9\uff1a<\/h4>\n<ol>\n<li>\u68af\u5ea6\u6d4b\u8bd5\u67d3\u6599\u6d53\u5ea6\uff0c\u5728\u65e0\u660e\u663e\u7ec6\u80de\u6bd2\u6027\u60c5\u51b5\u4e0b\uff0c\u5c3d\u53ef\u80fd\u589e\u52a0\u67d3\u8272\u6d53\u5ea6\u548c\u65f6\u95f4\uff1b<\/li>\n<li>\u4f7f\u7528\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u67d3\u8272\uff0c\u907f\u514d\u8840\u6e05\u5438\u9644\u67d3\u6599\uff0c\u964d\u4f4e\u6709\u6548\u67d3\u8272\u6d53\u5ea6\uff1b<\/li>\n<li>\u4f7f\u7528\u6807\u8bb0\u8f85\u52a9\u5242\u589e\u52a0\u6807\u8bb0\u6548\u7387\uff0c\u6291\u5236\u79bb\u5b50\u6cf5\u5916\u6392\u7ec6\u80de\u5185\u7684\u67d3\u6599\uff1b<\/li>\n<li>\u67d3\u8272\u5b8c\u6bd5\u540e\u4f7f\u7528\u5b8c\u5168\u57f9\u517b\u57fa\u57f9\u517b&#8221;\u6e05\u6d17&#8221;\u67d3\u6599 6-12 \u5c0f\u65f6\uff0c\u4f7f\u7ec6\u80de\u5410\u51fa\u80de\u5185\u591a\u4f59\u67d3\u6599\uff1b<\/li>\n<li>\u7ec6\u80de\u72b6\u6001\u548c\u6d3b\u6027\u6781\u5176\u91cd\u8981\uff0c\u9700\u8981\u9075\u5faa\u7ec6\u80de\u57f9\u517b\u539f\u5219\u517b\u51fa\u9ad8\u6d3b\u529b\u7684\u7ec6\u80de\u3002<\/li>\n<\/ol><\/div>\n<div class=\"hx-block\">\n<h4>\u2728 \u6210\u50cf\u8981\u70b9\uff1a<\/h4>\n<ol>\n<li>\u9009\u62e9\u6241\u5e73\u5706\u6247\u5f62\u7684\u7ec6\u80de\uff0c\u7ec6\u80de\u5b58\u5728\u5f02\u8d28\u6027\uff0c\u6559\u79d1\u4e66\u822c\u7684\u8f90\u5c04\u72b6\u7ec6\u80de\uff0c\u5728\u4e00\u4e9b\u7ec6\u80de\u4e2d\u901a\u5e38\u4ec5\u5360 5%-7%\uff0c\u7cbe\u5fc3\u6311\u9009\u89c6\u91ce\uff0c\u4f46\u662f\u5728\u7ec6\u80de\u9ad8\u6d3b\u529b\u7684\u60c5\u51b5\u4e0b\uff0c\u8be5\u6bd4\u4f8b\u4f1a\u5927\u5e45\u63d0\u5347\u3002<\/li>\n<li>\u6d3b\u7ec6\u80de\u62cd\u6444\u8981\u7535\uff0c\u9700\u8981\u68af\u5ea6\u6d4b\u8bd5\u66dd\u5149\u6761\u4ef6\uff0c\u5728 SNR 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