{"id":1576,"date":"2026-07-25T13:47:53","date_gmt":"2026-07-25T13:47:53","guid":{"rendered":"https:\/\/www.hxicollege.com\/?p=1576"},"modified":"2026-07-26T23:20:02","modified_gmt":"2026-07-26T15:20:02","slug":"biologist-microscope-1576","status":"publish","type":"post","link":"https:\/\/www.hxicollege.com\/index.php\/2026\/07\/25\/biologist-microscope-1576\/","title":{"rendered":"\u3010\u6280\u6cd5\u3011\u542c\u751f\u7269\u5b66\u5bb6\u8bf4\uff1a\u5982\u4f55\u9009\u62e9\u4e00\u6b3e\u663e\u5fae\u955c"},"content":{"rendered":"<style>\n.hx-article{<br \/>\n  max-width:1080px;margin:0 auto;padding:40px 28px 64px;<br \/>\n  font-family:-apple-system,BlinkMacSystemFont,\"Segoe UI\",\"PingFang SC\",\"Hiragino Sans GB\",\"Microsoft YaHei\",sans-serif;<br \/>\n  color:#1f2328;line-height:1.85;font-size:16px;<br \/>\n}<br \/>\n.hx-article h1{font-size:28px;font-weight:800;line-height:1.4;margin:0 0 18px;text-align:center;color:#0f172a;padding-bottom:14px;border-bottom:1px solid #e5e7eb;}<br \/>\n.hx-article h2{font-size:22px;font-weight:700;line-height:1.4;margin:36px 0 14px;color:#0f172a;}<br \/>\n.hx-article h3{font-size:18px;font-weight:700;margin:24px 0 10px;color:#1f2937;}<br \/>\n.hx-article p{margin:10px 0;}<br \/>\n.hx-article img{display:block;max-width:100%;height:auto;margin:18px auto;border-radius:8px;box-shadow:0 1px 3px rgba(15,23,42,.05),0 0 0 1px #eef0f3;}<br \/>\n.hx-article ul,.hx-article ol{margin:12px 0 18px;padding-left:24px;}<br \/>\n.hx-article blockquote{margin:18px 0;padding:14px 18px;border-left:4px solid #2563eb;background:#f8fafc;color:#475569;border-radius:4px;}<br \/>\nbody.aft-dark-mode .hx-article,body.aft-dark-mode .hx-article p{color:#e2e8f0;}<br \/>\nbody.aft-dark-mode .hx-article h1,body.aft-dark-mode .hx-article h2{color:#f1f5f9;}<br \/>\nbody.aft-dark-mode .hx-article h3{color:#cbd5e1;}<br \/>\nbody.aft-dark-mode .hx-article img{box-shadow:0 1px 3px rgba(0,0,0,.3),0 0 0 1px #334155;}<br \/>\nbody.aft-dark-mode .hx-article blockquote{background:#1e293b;border-color:#3b82f6;color:#cbd5e1;}<br \/>\n@media (max-width:780px){.hx-article{padding:24px 16px 48px;}.hx-article h1{font-size:22px;}}<br \/>\n<\/style>\n<div class=\"hx-article\" style=\"text-align: center;\">\n<section>\u672c\u6587\u7d20\u6750\u6765\u81eaibiology \u7cfb\u5217\u8bfe\u7a0b\uff0cstar cellbio\uff0cEMBL\uff0cAllen istitute\u200d\u200d\u548c\u5e73\u65f6\u7684\u79ef\u7d2f\u3002<\/section>\n<section>PS\uff1aibiology\u662f\u751f\u7269\u5b66\u5b66\u751f\u7684\u6bbf\u5802\uff0c\u6b22\u8fce\u5173\u6ce8ibiology\uff5e<\/section>\n<section>\u672c\u6587\u7684\u4e3b\u8981\u8109\u7edc\u662f\u5927\u4f19\u5fc3\u76ee\u4e2d\u7684\u5927\u751f\u7269\u5b66\u5bb6Ron Vale\u7684ibio\u8bfe\u7a0b\uff0c\u89e3\u51b3\u95ee\u9898\u7684\u601d\u8def\u6e05\u6670\u5230\u4e0d\u884c\uff01\u6211\u4eec\u5c31\u6765\u542c\u751f\u7269\u5b66\u5bb6Ron\u8bf4\u8bf4\uff0c\u5982\u4f55\u9009\u62e9\u4e00\u6b3e\u663e\u5fae\u955c\u3002\ud83d\udd2c<\/section>\n<section>\u672c\u6587\u7684\u6838\u5fc3\u5173\u952e\u8bcd\u6709\u4e09\u4e2a\uff1a\u89e3\u51b3\u95ee\u9898 \u89e3\u51b3\u95ee\u9898\u00a0\u89e3\u51b3\u95ee\u9898<\/section>\n<section><strong>\u76ee\u5f55\uff1a<\/strong><\/section>\n<section><strong>Part I\u00a0 \u00a0 \u9996\u5148\uff0c\u4f60\u8981\u95ee\u81ea\u5df1<\/strong><\/section>\n<section><strong>Part II\u00a0 \u00a0\u662f\u5426\u9700\u8981\u8367\u5149\u663e\u5fae\u955c<\/strong><\/section>\n<section><strong>Part III\u00a0 \u5982\u679c\u9009\u4e86\u8367\u5149\u663e\u5fae\u955c&#8230;<\/strong><\/section>\n<section><strong>Part\u00a0VI \u4f60\u6240\u5173\u6ce8\u7684\uff0c\u4e0d\u4ec5\u662f\u8367\u5149<\/strong><\/section>\n<section><strong>PartI \u9996\u5148\uff0c\u4f60\u9700\u8981\u95ee\u81ea\u5df1<\/strong><\/section>\n<section>\u751f\u7269\u5b66\u5bb6\u603b\u662f\u4f1a\u4ece\u95ee\u9898\u51fa\u53d1\uff0c\u8fd9\u662f\u4e00\u4e2a\u6781\u5176\u826f\u597d\u7684\u601d\u8003\u4e60\u60ef\u3002\u90a3\u5728\u771f\u6b63\u6d4f\u89c8\u663e\u5fae\u955c\u4e4b\u524d\uff0c\u4f60\u9700\u8981\u95ee\u81ea\u5df1\u51e0\u4e2a\u95ee\u9898\uff1a<\/section>\n<section><strong>1\uff0cwhat do you hope to learn?<\/strong><\/section>\n<section>\u4f60\u60f3\u8981\u56de\u7b54\u4ec0\u4e48\u95ee\u9898\uff1f\u4f60\u9700\u8981\u4ec0\u4e48\u6837\u7684\u6210\u50cf\u6570\u636e\u3002\u8fd9\u662f\u505a\u51b3\u5b9a\u6700\u91cd\u8981\u7684\u90e8\u5206\u3002<\/section>\n<section><strong>2\uff0cWhat do you have available?<\/strong><\/section>\n<section>\u4f60\u7684\u5b9e\u9a8c\u5ba4\u5e73\u53f0\u6709\u4ec0\u4e48\u8bbe\u5907\uff1f\u624b\u8fb9\u6709\u7684\u8bbe\u5907\u4f1a\u5e2e\u52a9\u4f60\u89e3\u51b3\u5b9e\u9645\u95ee\u9898\u3002<\/section>\n<section><strong>3\uff0cHow can you quantitate your images?<\/strong><\/section>\n<section>\u4f60\u8be5\u5982\u4f55\u5206\u6790\u4f60\u7684\u6570\u636e\u3002\u4eba\u4eec\u65e9\u5df2\u4e0d\u6ee1\u8db3\u4e8e\u4ec5\u4ec5\u62cd\u51fa\u6f02\u4eae\u56fe\u50cf\u800c\u5df2\uff0c\u56fe\u50cf\u4e2d\u5185\u6db5\u5927\u91cf\u7684\u4fe1\u606f\uff0c\u8fd9\u4e9b\u4fe1\u606f\u7684\u63d0\u53d6\u5bf9\u56de\u7b54\u751f\u7269\u95ee\u9898\u5c24\u5176\u91cd\u8981\u3002<\/section>\n<section><strong>\u4e0b\u9762\u7684\u7ae0\u8282\uff0c\u5c06\u4f1a\u51fa\u73b0\u5f88\u597d\u7684\u7814\u7a76\u5b9e\u4f8b\u3002<\/strong><\/section>\n<section><strong>Part II \u662f\u5426\u9700\u8981\u8367\u5149\u663e\u5fae\u955c<\/strong><\/section>\n<section><strong>\u900f\u5c04\u5149\u663e\u5fae\u955c\u548c\uff08\u53cd\u5c04\uff09\u8367\u5149\u663e\u5fae\u955c<\/strong><\/section>\n<section>\u4e00\u5f20\u56fe\u89e3\u91ca\u4ec0\u4e48\u4e8b\u900f\u5c04\u663e\u5fae\u955c\u548c\u8367\u5149\u663e\u5fae\u955c<\/section>\n<section><img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1576\/02.webp\" \/><\/section>\n<section>\n<section>\n<section>\n<section>\u00a0<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section>Transmitted light microscopy\u5149\u6e90\u7a7f\u900f\u6837\u54c1\u76f4\u63a5\u8fdb\u5165\u773c\u775b<\/section>\n<section>Good for measuring cell behavior~<\/section>\n<section>\u9002\u5408\u5206\u6790\u7ec6\u80de\u7684\u8868\u578b\uff0c\u4e0d\u9700\u8981\u57fa\u56e0\u7f16\u8f91\u8367\u5149\uff0c\u6bd4\u8367\u5149\u6210\u50cf\u66f4\u5c11\u7684\u5149\u635f\u4f24\u3002<\/section>\n<section>fluorescence\u00a0microscopy\u7684\u5149\u6e90\u7167\u5c04\u6837\u54c1\u6fc0\u53d1\u6837\u54c1\u7ecf\u8fc7\u53cd\u5c04\u8fdb\u5165\u773c\u775b<\/section>\n<section>Good for measuring molecular behavior~<\/section>\n<section>\u9002\u5408\u7814\u7a76\u5206\u5b50\u7684\u884c\u4e3a<\/section>\n<section>\u6b64\u5916\uff0c\u4f60\u5e76\u4e0d\u603b\u662f\u9700\u8981\u5f88\u8d35\u7684\u663e\u5fae\u955c\u624d\u80fd\u89e3\u51b3\u95ee\u9898\u3002<\/section>\n<section><strong>\u4ee5\u4e0b\u662f\u4e24\u4e2a\u8d85\u4fbf\u5b9c\u663e\u5fae\u955c\u5b8c\u7f8e\u89e3\u51b3\u95ee\u9898\u7684\u4f8b\u8bc1\uff1a<br \/>\n<\/strong><\/section>\n<section><\/section>\n<section><\/section>\n<section><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1576\/04.gif\" width=\"524\" height=\"393\" \/><\/section>\n<section><\/section>\n<section><strong>\u5c0f\u7f16\u81ea\u5df1\u7528\u660e\u573a\u663e\u5fae\u955c\u62cd\u6444\u7684\u4f24\u53e3\u6108\u5408\u8fc7\u7a0b\u7684\u7ec6\u80de\u8fc1\u79fb \uff08Hao et al, EMBO Rep. e48385\uff09<\/strong><\/section>\n<section><\/section>\n<section><\/section>\n<section><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1576\/05.webp\" width=\"523\" height=\"392\" \/><\/section>\n<section><\/section>\n<section><strong style=\"letter-spacing: 0.05em;\">\u7814\u7a76\u7ec6\u80de\u8fc1\u79fb\u7684\uff0c\u7814\u7a76\u5fae\u7ba1\u7684\u6697\u573a\u663e\u5fae\u955c\uff08Horio and Hotani, Nature 321:605-7,1986\uff09<\/strong><\/section>\n<section><\/section>\n<\/div>\n<section><\/section>\n<section><\/section>\n<div class=\"hx-article\" style=\"text-align: center;\">\n<section><\/section>\n<section><strong><strong>Part III\u00a0 \u5982\u679c\u9009\u4e86\u8367\u5149\u663e\u5fae\u955c&#8230;<\/strong><\/strong><\/section>\n<section>\u5982\u679c\u4f60\u9009\u62e9\u4e86\u8367\u5149\u663e\u5fae\u955c\uff0c\u4f60\u4ecd\u7136\u9700\u8981\u95ee\u81ea\u5df1\uff1a<\/section>\n<section><strong>1\uff0cAre you looking at a thick or thin specimen?\u00a0<\/strong><\/section>\n<section>\u4f60\u662f\u5728\u770b\u539a\u7684\u8fd8\u662f\u8584\u7684\u6837\u54c1\uff1f(\u6210\u50cf\u6df1\u5ea6)<\/section>\n<section><strong>2\uff0cAre you looking at a living sample or a fixed sample?\u00a0<\/strong><\/section>\n<section>\u4f60\u662f\u5728\u770b\u6d3b\u7ec6\u80de\u8fd8\u662f\u56fa\u5b9a\u7ec6\u80de\uff1f\uff08\u91c7\u6837\u7387\u548c\u7ec6\u80de\u635f\u4f24\uff09<\/section>\n<section><strong>Epifluorescence\u00a0<\/strong><\/section>\n<section><strong>\u5bbd\u573a\u663e\u5fae\u955c<\/strong><\/section>\n<section>\u4f18\u70b9\uff1a<\/section>\n<section>low cost (no laser)\u00a0\u6210\u672c\u4f4e\uff08\u6728\u6709\u6fc0\u5149\uff09<\/section>\n<section>Easy to use \u5bb9\u6613\u7528\uff0c\u5927\u591a\u6570\u5b9e\u9a8c\u5ba4\u5f88\u5bb9\u6613\u914d\u5907<\/section>\n<section>\u7f3a\u70b9\uff1a<\/section>\n<section>Lower SNR (out of focus fluorescence) \u4fe1\u566a\u6bd4\u8f83\u4f4e\uff08\u56e0\u4e3a\u6709\u805a\u7126\u9762\u4e4b\u5916\u7684\u8367\u5149\uff09<\/section>\n<section>More damaging to live cells. \u5bf9\u6d3b\u7ec6\u80de\u4f1a\u9020\u6210\u8f83\u5927\u635f\u4f24<\/section>\n<section>\u5373\u4f7f\u73b0\u5728\u5404\u79cd\u9ad8\u7aef\u7684\u663e\u5fae\u955c\u5f00\u59cb\u6ee1\u5929\u98de\uff0c\u50cf\u8fd9\u6837\u7684\u663e\u5fae\u955c\u6709\u5f88\u597d\u7684\u7528\u6b66\u4e4b\u5730\uff1a<\/section>\n<section>\u6bd4\u5982\uff1aGood for 96-well KD screen~\u00a0<\/section>\n<section>\u6b64\u65f6\u4f60\u53ea\u9700\u8981\u4e00\u6807\u8bb0\u7b80\u5355\u7684\u8367\u5149\u4fe1\u53f7\uff0c\u4f7f\u7528\u6700\u591a40X\u7684\u7269\u955c\u6765\u8fdb\u884c\u6709\u548c\u65e0\u7684\u68c0\u6d4b\uff0c\u5c31\u53ef\u4ee5\u6572\u4f4e\u662f\u5426\u6709\u6548\u679c\u7684\u7ed3\u8bba\u3002<\/section>\n<section><img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1576\/06.webp\" \/><\/section>\n<section>\u7528\u4e8e\u7b5b\u9009\u768496\u5b54\u677f<\/section>\n<section><strong>Deconvolution microscopy<\/strong><\/section>\n<section><strong>\u53bb\u5377\u79ef\u663e\u5fae\u955c<\/strong><\/section>\n<section>\u8fd9\u79cd\u663e\u5fae\u955c\u6280\u672f\u662f\u901a\u8fc7\u8ba1\u7b97\u8fc7\u7a0b\uff0c\u8fbe\u5230\u53bb\u9664\u79bb\u5f00\u7126\u5e73\u9762\u7684\u5149\u662f\u6548\u679c<\/section>\n<section>\u5bf9\u4e00\u822c\u7684\u5b9e\u9a8c\u5ba4\u6765\u8bf4\u4e5f\u662f\u5f88\u597d\u642d\u5efa\uff0c\u786c\u4ef6\u4e0a\u53ea\u9700\u8981\u5bbd\u573a\u8367\u5149\u663e\u5fae\u955c+Z stepper\u3002\u8fd9\u4e24\u4e2a\u4e00\u822c\u663e\u5fae\u955c\u662f\u914d\u5907\u7684\u3002\u56e0\u6b64\u53ea\u9700\u8981\u8d2d\u4e70\u7528\u4e8e\u53bb\u5377\u79ef\u7684\u8f6f\u4ef6\u3002<\/section>\n<section><img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1576\/07.webp\" \/><\/section>\n<section>\u53bb\u5377\u79ef\u524d\u548c\u53bb\u5377\u79ef\u540ehttps:\/\/cam.facilities.northwestern.edu\/588-2\/deconvolution\/<\/section>\n<section><strong>Laser-based sectioning microscopy<\/strong><\/section>\n<section><strong>\u57fa\u4e8e\u6fc0\u5149\u7684\u5207\u7247\u663e\u5fae\u955c<\/strong><\/section>\n<section>\u5982\u679c\u4ee5\u4e0a\u663e\u5fae\u955c\u4e0d\u80fd\u6ee1\u8db3\u4e8e\u4f60\u7684\u9700\u6c42\uff0c\u90a3\u4e48\u9700\u8981\u6c42\u52a9\u4e8e\u66f4\u9ad8\u7ea7\u7684\u663e\u5fae\u955c\u3002\u8fd9\u4e00\u90e8\u5206\u8bb2\u57fa\u4e8e\u6fc0\u5149\u7684\u5207\u7247\u663e\u5fae\u955c\u3002<\/section>\n<section>\u9996\u5148\uff0c\u6839\u636e\u4f60\u8981\u7814\u7a76\u7684\u95ee\u9898\uff0c\u4f60\u7684\u88ab\u7814\u7a76\u7269\u53ef\u80fd\u6709\u4ee5\u4e0b\u51e0\u79cd\u7c7b\u578b\uff1a<\/section>\n<section><strong>i\uff09\u00a0\u88ab\u6210\u50cf\u7269\u5728\u73bb\u7483\u8868\u9762\uff1bii\uff09\u6837\u54c1\u539a\u5ea6\u5c0f\u4e8e100um\uff1biii\uff09\u6837\u54c1\u539a\u5ea6\u5927\u4e8e100um<\/strong><\/section>\n<section><strong>i) Object near the coverslip surface:\u00a0<\/strong><\/section>\n<section>\u88ab\u6210\u50cf\u7269\u5728\u73bb\u7483\u8868\u9762<\/section>\n<section>TIRF, total internal reflection fluorescence<\/section>\n<section>\u6b64\u65f6\u4f60\u53ef\u4ee5\u9009\u62e9\u5168\u5185\u53cd\u5c04\u663e\u5fae\u955c\uff0c\u4e00\u4e2a\u5fae\u4e1d\u6210\u50cf\u7684\u4f8b\u5b50<\/section>\n<section><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1576\/08.webp\" width=\"473\" height=\"305\" \/><\/section>\n<section><\/section>\n<section>\u5fae\u4e1d\u7684\u5bbd\u573a\u6210\u50cf\u548cTIRF\u6210\u50cf\u00a0https:\/\/www.flickr.com\/photos\/mcammer\/267971287<\/section>\n<section>\u6211\u4eec\u7684\u89c2\u6d4b\u624b\u6bb5\u4ece\u6765\u4e0d\u53ea\u5c40\u9650\u4e8e\u5149\u5b66\u4fe1\u53f7\uff0c\u4e0b\u9762\u662f\u4e00\u4e9b\u5f88creative\u7684\u65b9\u6cd5\u5b66\u3002<\/section>\n<section>TIRF\u6210\u50cf\u7528\u4e8e\u86cb\u767d\u7ec4\u7814\u7a76\uff1a\u6297\u4f53\u7c98\u9644\u4e8e\u73bb\u7483\u8868\u9762\uff0cpull down\u7ec6\u80de\u5185\u7684\u86cb\u767d\u590d\u5408\u7269<\/section>\n<section><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1576\/09.webp\" width=\"496\" height=\"190\" \/><\/section>\n<section><\/section>\n<section><\/section>\n<section><\/section>\n<section><\/section>\n<section><\/section>\n<section><\/section>\n<section><\/section>\n<section><\/section>\n<section><\/section>\n<section><\/section>\n<section>single molecule immunoprecipitation SiMPull assay,\u00a02011 nature jain et al.<\/section>\n<section>TIRF\u6210\u50cf\u7528\u4e8e\u6d4b\u5e8f\u6280\u672f\uff1a<\/section>\n<section><img loading=\"lazy\" decoding=\"async\" class=\"\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1576\/10.webp\" width=\"507\" height=\"256\" \/><\/section>\n<section>new\u00a0DNA sequencing technologies\u00a0also use TIRF imaging \uff08ibiology course\uff09<\/section>\n<section><strong>ii) &lt;100 um thick (cell):<\/strong><\/section>\n<section>\u88ab\u6210\u50cf\u7269\u7684\u539a\u5ea6\u5c0f\u4e8e100 um\uff0c\u4f7f\u7528pin-hole microscopy\u00a0<\/section>\n<section>\u9488\u5b54\u663e\u5fae\u955c\uff0c\u70b9\u72b6\u6216\u8005\u7ebf\u72b6\u7684\u626b\u63cf\uff0c\u6216\u8005\u8f6c\u76d8\u5171\u805a\u7126\u663e\u5fae\u955c\u90fd\u6bd4\u8f83\u9002\u5408\u8fd9\u4e2a\u539a\u5ea6\u3002<\/section>\n<section>\u8f6c\u76d8\u5171\u805a\u7126\u663e\u5fae\u955cversus\u70b9\u626b\u63cf\u663e\u5fae\u955c<\/section>\n<section>Spinning disk confocal\u00a0<\/section>\n<section>\u5bf9\u6d3b\u7ec6\u80de\u6210\u50cf\u53cb\u597d\uff0c\u53ef\u4ee5\u6709\u66f4\u5feb\u7684\u91c7\u6837\u9891\u7387<\/section>\n<section>\u7814\u7a76\u6d3b\u7ec6\u80de\uff0c\u52a8\u6001\u95ee\u9898\uff0c\u5feb\u901f\u8fd0\u52a8\u7684\u9996\u9009<\/section>\n<section>Point scanning confocal (&lt;100um)<\/section>\n<section>\u5bf9\u56fa\u5b9a\u6837\u54c1\u53cb\u597d\uff0c\u8fd9\u79cd\u6837\u54c1\u4e0d\u592a\u9700\u8981\u8003\u8651\u5149\u635f\u4f24\u3002<\/section>\n<section>\u884d\u751f\u51fa\u7ed3\u5408\u6280\u672f\uff1a\u57fa\u4e8ephotobleaching\u7684\u7814\u7a76\u5206\u5b50dynamic\u7684\u6280\u672f\u3002<\/section>\n<section><strong>Light Sheet microscopy<\/strong><\/section>\n<section><strong>\u5149\u6bef\u663e\u5fae\u955c<\/strong><\/section>\n<section>light sheet\u626b\u63cf\u793a\u610f\u56fe<\/section>\n<section>\u9002\u5408\u7528\u4e8e\u6591\u9a6c\u9c7c\u80da\u80ce\u7684\u6210\u50cf, \u56fe\u50cf\u6765\u81eaEMBL center<\/section>\n<section>lightaheet\u7684\u4f18\u70b9\u662f\uff0c\u4f4e\u5149\u635f\u4f24\uff0c\u66f4\u5feb\u7684\u91c7\u96c6\u901f\u7387\uff0c\u6240\u4ee5\u5f88\u9002\u5408\u80da\u80ce\u53d1\u80b2\u7684\u7814\u7a76\u3002<\/section>\n<section><strong>iii) &gt;100 um thick (tissue):<\/strong><\/section>\n<section>\u88ab\u6210\u50cf\u7269\u7684\u539a\u5ea6\u5927\u4e8e100um<\/section>\n<section>Multi-photon microscopy and array tomography<\/section>\n<section>\u53cc\u5149\u5b50\u663e\u5fae\u955c<\/section>\n<section>\u6210\u50cf\u6df1\u5ea6\u589e\u52a0\uff0c\u00a0\u5982100\uff5e500um \u4f7f\u7528\u53cc\u5149\u5b50\u663e\u5fae\u955c\u3002<\/section>\n<section>\u5bf9\u4e8e\u6781\u5176\u539a\u7684\u6837\u54c1\uff0c\u8981\u8003\u8651\u7269\u7406\u5207\u5272\u7684\u65b9\u6cd5array tomography\u3002<\/section>\n<section>\u5982\u6574\u4e2a\u8111\uff0c\u4f7f\u7528\u7ed3\u5408\u7269\u7406\u5207\u5272\u7684\u663e\u5fae\u955c\uff1a<\/section>\n<section><img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1576\/11.webp\" \/><\/section>\n<section>\u94bb\u77f3\u5200\u5207\u5272\u7684\u8111\u5207\u7247\u8584\u7247Allen Institute\u00a0<\/section>\n<section>\u9f20\u8111\u7684\u4e91\u56fe-Allen Institute\u00a0<\/section>\n<section><strong>\u00a0Super-resolution microscopy<\/strong><\/section>\n<section><strong>\u8d85\u5206\u8fa8\u663e\u5fae\u955c<\/strong><\/section>\n<section>\u5982\u679c\u4f60\u6709\u4e00\u4e2a\u95ee\u9898\uff0c\u6781\u597d\u7684\u5339\u914d\u8fd9\u4e9b<strong>\u5206\u8fa8\u7387<\/strong>\uff0c\u663e\u5fae\u955c\u4f1a\u662f\u4e00\u4e2a\u89e3\u51b3\u95ee\u9898\u7684\u597d\u5de5\u5177\u3002<\/section>\n<section>\u6211\u4eec\u6765\u770b\u770b\u51e0\u79cd\u8457\u540d\u7684\u8d85\u5206\u8fa8\u663e\u5fae\u955c\u9002\u914d\u7684\u5206\u8fa8\u7387\u662f\u591a\u5c11\uff1a<\/section>\n<section>Conventional\uff0c\u4f20\u7edf\u7684\u663e\u5fae\u6280\u672f: 200~250 nm at the best<\/section>\n<section>Structured illumination\uff0c\u7ed3\u6784\u5149\u7167\u660e\uff1a100nm, live imaging<\/section>\n<section>STORM PALM STED \u8d85\u5206\u8fa8\u663e\u5fae\u955c\uff1a\uff5e40nm most powerful for fixed samples.<\/section>\n<section>\u4e3a\u4e86\u505a\u6bd4\u8f83\uff0c\u51b7\u51bb\u7535\u955cCryo-EM \u7684\u5206\u8fa8\u73872~4 nm frozen samples unstained complex\u3002\u73b0\u5728\u8fd8\u6709\u7ed3\u5408\u51b7\u51bb\u7535\u955c\u4e8e\u8d85\u5206\u8fa8\u8367\u5149\u6210\u50cf\u6280\u672f-\u5149\u7535\u8054\u5408\u6280\u672f\u3002<\/section>\n<section>\u4ee5\u4e0b\u6709\u4e00\u4e2a\u4f8b\u5b50<strong>\u5f70\u663e\u4e86\u751f\u7269\u5b66\u5bb6\u654f\u9510\u7684insight<\/strong>\u3002\u4ed6\u8981\u7814\u7a76\u7684\u95ee\u9898\u672c\u8eab\uff0c\u5f88\u597d\u7684\u543b\u5408\u4e86\u4ed6\u6240\u9009\u62e9\u7684\u6280\u672f\u7684\u5206\u8fa8\u7387\u3002<\/section>\n<section>SIM\u4e2d\u5fc3\u7c92\u7684\u7814\u7a76\u3002<\/section>\n<section><img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1576\/12.webp\" \/><\/section>\n<section>SIM\u7814\u7a76\u4e0d\u540c\u7684\u4e2d\u5fc3\u7c92\u86cb\u767d\u7684\u5f62\u8c8c\u7279\u5f81\uff0cMennella et al, NCB 2012.<\/section>\n<section><strong>Part\u00a0VI \u4f60\u6240\u5173\u6ce8\u7684\uff0c\u4e0d\u4ec5\u662f\u8367\u5149<\/strong><\/section>\n<section><strong>More than just optics<\/strong><\/section>\n<section><strong>\u4e0d\u4ec5\u662f\u8367\u5149<\/strong><\/section>\n<section><strong>\u65b0\u7684\u63a2\u9488\u6765\u68c0\u6d4b\u7ec6\u80de\u5185\u7684\u8fc7\u7a0b<\/strong><\/section>\n<section><img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1576\/13.webp\" \/><\/section>\n<section>voltage-sensitive microbial rhodopsin. \u5927\u9f20\u795e\u7ecf\u5143\u4e2d\u7684\u52a8\u4f5c\u7535\u4f4d\u3002\u00a0\u00a0<\/section>\n<section>Kralj et al, nature methods 2011.<\/section>\n<section>\u7528\u5149\u505a\u5de5\u5177\uff1a<\/section>\n<section><img loading=\"lazy\" decoding=\"async\" class=\"\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1576\/14.gif\" width=\"602\" height=\"200\" \/><\/section>\n<section>\u5c0f\u7f16\u81ea\u5df1\u62cd\u6444\u7684\u7528\u5149\u9057\u4f20\u5de5\u5177\u63a7\u5236\u7ec6\u80de\u8fc1\u79fb\u200d<\/section>\n<section>\u6211\u7684\u4e13\u4e1a\u5708\u5185\u5927\u591a\u6570\u90fd\u662f\u6bd4\u6211\u6c34\u5e73\u9ad8\u7684\u4eba\uff0c\u5199\u8fd9\u7bc7\u6587\u7ae0\uff0c\u4e5f\u7b97\u7ed9\u522b\u4eba\u4e00\u4e2a\u8ba8\u8bba\u7684\u9776\u70b9\uff0c\u5e2e\u52a9\u81ea\u5df1\u67e5\u6f0f\u8865\u7f3a\u4e86\u3002<\/section>\n<section>\u200d<\/section>\n<section>\n<section>\u2014\u2014\u2014\u2014\u00a0\u00a0e n d\u00a0\u2014\u2014\u2014\u2014<\/section>\n<\/section>\n<section>\u200d\u200d<\/section>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u672c\u6587\u7d20\u6750\u6765\u81eaibiology \u7cfb\u5217\u8bfe\u7a0b\uff0cstar cellbio\uff0cEMBL\uff0cAllen istitute\u200d\u200d\u548c\u5e73\u65f6\u7684\u79ef\u7d2f\u3002<br \/>\nPS\uff1aibiology\u662f\u751f\u7269\u5b66\u5b66\u751f\u7684\u6bbf\u5802\uff0c\u6b22\u8fce\u5173\u6ce8ibiology\uff5e<br \/>\n\u672c\u6587\u7684\u4e3b\u8981\u8109\u7edc\u662f\u5927\u4f19\u5fc3\u76ee\u4e2d\u7684\u5927\u751f\u7269\u5b66\u5bb6Ron Vale\u7684ibio\u8bfe\u7a0b\uff0c\u89e3\u51b3\u95ee\u9898\u7684\u601d\u8def\u6e05\u6670\u5230\u4e0d\u884c\uff01\u6211\u4eec\u5c31\u6765\u542c\u751f\u7269\u5b66\u5bb6Ron\u8bf4\u8bf4\uff0c\u5982\u4f55\u9009\u62e9\u4e00\u6b3e\u663e\u5fae\u955c\u3002\ud83d\udd2c<br \/>\n\u672c\u6587\u7684\u6838\u5fc3\u5173\u952e\u8bcd\u6709\u4e09\u4e2a\uff1a\u89e3\u51b3\u95ee\u9898 \u89e3\u51b3\u95ee\u9898\u00a0\u89e3\u51b3\u95ee\u9898<br \/>\n\u76ee\u5f55\uff1a<\/p>\n<p>Part I\u00a0 \u00a0 \u9996\u5148\uff0c\u4f60\u8981\u95ee\u81ea\u5df1<br \/>\nPart II\u00a0 \u00a0\u662f\u5426\u9700\u8981\u8367\u5149\u663e\u5fae\u955c<br \/>\nPart III\u00a0 \u5982\u679c\u9009\u4e86\u8367\u5149\u663e\u5fae\u955c&#8230;<br \/>\nPart\u00a0VI \u4f60\u6240\u5173\u6ce8\u7684\uff0c\u4e0d\u4ec5\u662f\u8367\u5149<br \/>\nPartI \u9996\u5148\uff0c\u4f60\u9700\u8981\u95ee\u81ea\u5df1<br \/>\n\u751f\u7269\u5b66\u5bb6\u603b\u662f\u4f1a\u4ece\u95ee\u9898\u51fa\u53d1\uff0c\u8fd9\u662f\u4e00\u4e2a\u6781\u5176\u826f\u597d\u7684\u601d\u8003\u4e60\u60ef\u3002\u90a3\u5728\u771f\u6b63\u6d4f\u89c8\u663e\u5fae\u955c\u4e4b\u524d\uff0c\u4f60\u9700\u8981\u95ee\u81ea\u5df1\u51e0\u4e2a\u95ee\u9898\u3002<\/p>\n","protected":false},"author":1,"featured_media":1587,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[54],"tags":[],"class_list":["post-1576","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-article-sharing"],"_links":{"self":[{"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/posts\/1576","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/comments?post=1576"}],"version-history":[{"count":15,"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/posts\/1576\/revisions"}],"predecessor-version":[{"id":1615,"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/posts\/1576\/revisions\/1615"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/media\/1587"}],"wp:attachment":[{"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/media?parent=1576"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/categories?post=1576"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.hxicollege.com\/index.php\/wp-json\/wp\/v2\/tags?post=1576"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}