{"id":1494,"date":"2026-07-24T13:44:07","date_gmt":"2026-07-24T13:44:07","guid":{"rendered":"https:\/\/www.hxicollege.com\/?p=1494"},"modified":"2026-07-25T00:43:54","modified_gmt":"2026-07-24T16:43:54","slug":"microfilaments-fine-detail-1494","status":"publish","type":"post","link":"https:\/\/www.hxicollege.com\/index.php\/2026\/07\/24\/microfilaments-fine-detail-1494\/","title":{"rendered":"\u4e3a\u4ec0\u4e48\u7ec6\u80de\u5fae\u4e1d\u603b\u662f\u6807\u4e0d\u5230\u7ec6\u8282\u7ed3\u6784\uff1f\u95ee\u9898\u5728\u8fd9\u91cc\uff01\uff08\u4e00\uff09"},"content":{"rendered":"<style>\n  \/* ===== \u6574\u7bc7\u5bb9\u5668\uff1a\u5bbd\u7248 ===== *\/\n  .hx-article{\n    max-width:1080px;\n    margin:0 auto;\n    padding:40px 28px 64px;\n    font-family:-apple-system,BlinkMacSystemFont,\"Segoe UI\",\"PingFang SC\",\"Hiragino Sans GB\",\"Microsoft YaHei\",sans-serif;\n    color:#1f2328;\n    line-height:1.85;\n    font-size:16px;\n  }\n  \/* ===== \u6807\u9898\u5c42\u7ea7\uff08h1 \u4e0d\u5165\u5185\u5bb9\uff0c\u7531 post_title \u6e32\u67d3\uff09 ===== *\/\n  .hx-article h2{\n    font-size:22px;\n    font-weight:700;\n    line-height:1.4;\n    margin:48px 0 20px;\n    padding:12px 18px;\n    background:linear-gradient(90deg,#f1f5f9,transparent 70%);\n    border-left:4px solid #2563eb;\n    border-radius:4px;\n    color:#0f172a;\n  }\n  .hx-article h3{\n    font-size:18px;\n    font-weight:700;\n    margin:28px 0 12px;\n    color:#1f2937;\n    display:flex;\n    align-items:center;\n    gap:8px;\n  }\n  .hx-article h3::before{\n    content:\"\";\n    flex:0 0 auto;\n    width:4px;\n    height:18px;\n    background:#2563eb;\n    border-radius:2px;\n  }\n  .hx-article h4{\n    font-size:15px;\n    font-weight:700;\n    margin:22px 0 10px;\n    color:#475569;\n    display:flex;\n    align-items:center;\n    gap:6px;\n  }\n  .hx-article h4 .ico{font-size:16px;}\n  .hx-article p{margin:8px 0;}\n  \/* ===== \u4ecb\u7ecd\u5361 ===== *\/\n  .hx-intro{\n    display:flex;\n    gap:28px;\n    align-items:stretch;\n    margin:28px 0 40px;\n    padding:24px;\n    background:linear-gradient(135deg,#f8fafc,#eef2f7);\n    border-radius:12px;\n  }\n  .hx-intro .info{flex:1;display:flex;flex-direction:column;justify-content:center;gap:12px;}\n  .hx-intro .img{\n    flex:0 0 38%;\n    border-radius:8px;\n    overflow:hidden;\n    background:#fff;\n    box-shadow:0 2px 8px rgba(15,23,42,.06);\n  }\n  .hx-intro .img img{display:block;width:100%;height:100%;object-fit:cover;}\n  \/* ===== \u56fe\u6587\u5e76\u6392\u5361 ===== *\/\n  .hx-pair{\n    display:flex;\n    gap:28px;\n    align-items:stretch;\n    margin:18px 0;\n    background:#fff;\n    border-radius:12px;\n    overflow:hidden;\n    box-shadow:0 1px 3px rgba(15,23,42,.05),0 0 0 1px #eef0f3;\n  }\n  .hx-pair.reverse{flex-direction:row-reverse;}\n  .hx-pair .img{\n    flex:0 0 46%;\n    background:#f8fafc;\n    display:flex;\n    align-items:center;\n    justify-content:center;\n    overflow:hidden;\n  }\n  .hx-pair .img img{display:block;width:100%;height:100%;object-fit:cover;}\n  .hx-pair .text{\n    flex:1;\n    display:flex;\n    flex-direction:column;\n    justify-content:center;\n    padding:22px 26px;\n  }\n  .hx-pair .text h3{margin-top:0;}\n  \/* ===== Callout \u5757 ===== *\/\n  .hx-callout{\n    margin:16px 0;\n    padding:14px 18px;\n    border-left:4px solid #64748b;\n    border-radius:6px;\n    font-size:15px;\n    line-height:1.85;\n  }\n  .hx-callout.info{background:#eff6ff;border-color:#3b82f6;color:#1e3a8a;}\n  .hx-callout.warn{background:#fff7ed;border-color:#f97316;color:#7c2d12;}\n  .hx-callout.tip {background:#f0fdf4;border-color:#22c55e;color:#14532d;}\n  .hx-callout.key {background:#faf5ff;border-color:#a855f7;color:#581c87;}\n  \/* ===== \u8868\u683c ===== *\/\n  .hx-table{\n    width:100%;\n    border-collapse:separate;\n    border-spacing:0;\n    margin:14px 0 22px;\n    font-size:14.5px;\n    background:#fff;\n    border-radius:8px;\n    overflow:hidden;\n    box-shadow:0 0 0 1px #e2e8f0;\n  }\n  .hx-table th,.hx-table td{\n    padding:11px 16px;\n    text-align:left;\n    border-bottom:1px solid #eef0f3;\n    vertical-align:top;\n  }\n  .hx-table tbody tr:last-child td{border-bottom:0;}\n  .hx-table thead th{\n    background:#f1f5f9;\n    font-weight:700;\n    color:#334155;\n    font-size:13.5px;\n    letter-spacing:.02em;\n  }\n  .hx-table tbody tr:nth-child(even) td{background:#fafbfc;}\n  \/* ===== \u6b65\u9aa4\u5c0f\u89d2\u6807 ===== *\/\n  .hx-badge{\n    display:inline-flex;\n    align-items:center;\n    justify-content:center;\n    min-width:34px;\n    height:24px;\n    padding:0 8px;\n    border-radius:6px;\n    background:#2563eb;\n    color:#fff;\n    font-weight:700;\n    font-size:12.5px;\n    margin-right:8px;\n    font-family:Menlo,Monaco,Consolas,monospace;\n    flex:0 0 auto;\n  }\n  .hx-badge small{color:#bfdbfe;font-weight:500;margin-left:4px;font-size:11px;}\n  \/* ===== \u5217\u8868 ===== *\/\n  .hx-list{margin:12px 0 18px;padding:0;list-style:none;}\n  .hx-list li{position:relative;padding:6px 0 6px 28px;line-height:1.85;}\n  .hx-list li::before{\n    content:\"\";\n    position:absolute;\n    left:8px;\n    top:16px;\n    width:7px;\n    height:7px;\n    border-radius:50%;\n    background:#2563eb;\n  }\n  \/* ===== \u89c6\u9891\u94fe\u63a5\u80f6\u56ca\u6309\u94ae ===== *\/\n  .hx-video-wrap{margin:8px 0 20px;display:flex;justify-content:center;}\n  .hx-video-btn{\n    display:inline-flex;align-items:center;gap:8px;\n    padding:12px 28px;border-radius:999px;\n    background:linear-gradient(135deg,#2563eb,#1d4ed8);\n    color:#fff !important;font-size:16px;font-weight:600;\n    text-decoration:none !important;\n    box-shadow:0 4px 14px rgba(37,99,235,.25),0 1px 3px rgba(15,23,42,.08);\n    transition:transform .15s ease,box-shadow .15s ease;\n  }\n  .hx-video-btn:hover{\n    transform:translateY(-1px);\n    box-shadow:0 6px 18px rgba(37,99,235,.35),0 2px 5px rgba(15,23,42,.1);\n    color:#fff !important;text-decoration:none !important;\n  }\n  .hx-video-btn:active{transform:translateY(0);}\n  \/* ===== \u5355\u56fe\u5361 ===== *\/\n  .hx-figure{\n    margin:18px 0;\n    background:#fff;\n    border-radius:12px;\n    overflow:hidden;\n    box-shadow:0 1px 3px rgba(15,23,42,.05),0 0 0 1px #eef0f3;\n  }\n  .hx-figure img{\n    display:block;\n    width:100%;\n    height:auto;\n    max-height:640px;\n    object-fit:contain;\n    margin:0 auto;\n  }\n  \/* ===== \u53cc\u5217\u56fe\u7ec4 ===== *\/\n  .hx-figure-grid{\n    display:grid;\n    grid-template-columns:1fr 1fr;\n    gap:10px;\n    margin:20px 0;\n  }\n  .hx-figure-grid .hx-figure{margin:0;}\n  .hx-figure-grid.single{grid-template-columns:1fr;max-width:50%;margin-left:auto;margin-right:auto;}\n  \/* ===== inline ===== *\/\n  code{\n    background:#f1f5f9;\n    padding:2px 7px;\n    border-radius:4px;\n    color:#c026d3;\n    font-family:Menlo,Monaco,Consolas,monospace;\n    font-size:.92em;\n  }\n  small{color:#64748b;font-size:.85em;}\n  \/* ===== \u6536\u5c3e ===== *\/\n  .hx-end{\n    text-align:center;\n    color:#94a3b8;\n    font-size:13px;\n    margin:56px 0 0;\n    padding-top:22px;\n    border-top:1px dashed #e2e8f0;\n  }\n  \/* ===== CelebNews \u591c\u95f4\u6a21\u5f0f\u9002\u914d ===== *\/\n  body.aft-dark-mode .hx-article,\n  body.aft-dark-mode .hx-article p{color:#e2e8f0;}\n  body.aft-dark-mode .hx-article h2{color:#f1f5f9;background:linear-gradient(90deg,#1e293b,transparent 70%);}\n  body.aft-dark-mode .hx-article h3{color:#f1f5f9;}\n  body.aft-dark-mode .hx-article h4{color:#cbd5e1;}\n  body.aft-dark-mode .hx-intro{background:linear-gradient(135deg,#1e293b,#0f172a);}\n  body.aft-dark-mode .hx-pair,\n  body.aft-dark-mode .hx-figure{background:#1e293b;box-shadow:0 1px 3px rgba(0,0,0,.3),0 0 0 1px #334155;}\n  body.aft-dark-mode .hx-pair .text,\n  body.aft-dark-mode .hx-intro .info{color:#e2e8f0;}\n  body.aft-dark-mode .hx-pair .img{background:#0f172a;}\n  body.aft-dark-mode .hx-callout{background:#1e293b;color:#e2e8f0;}\n  body.aft-dark-mode .hx-callout.info{background:#1e3a5f;color:#bfdbfe;border-color:#3b82f6;}\n  body.aft-dark-mode .hx-callout.warn{background:#5c2d12;color:#fed7aa;border-color:#f97316;}\n  body.aft-dark-mode .hx-callout.tip {background:#14532d;color:#bbf7d0;border-color:#22c55e;}\n  body.aft-dark-mode .hx-callout.key{background:#4c1d95;color:#e9d5ff;border-color:#a855f7;}\n  body.aft-dark-mode .hx-table{background:#1e293b;color:#e2e8f0;}\n  body.aft-dark-mode .hx-table th{background:#0f172a;color:#f1f5f9;}\n  body.aft-dark-mode .hx-table td{border-color:#334155;color:#cbd5e1;}\n  body.aft-dark-mode .hx-table tbody tr:nth-child(even) td{background:#172033;}\n  body.aft-dark-mode .hx-list li{color:#e2e8f0;}\n  body.aft-dark-mode code{background:#1e293b;color:#f472b6;}\n  body.aft-dark-mode .hx-end{color:#64748b;border-color:#334155;}\n  \/* ===== \u79fb\u52a8\u7aef\u56de\u9000 ===== *\/\n  @media (max-width:780px){\n    .hx-intro,.hx-pair,.hx-pair.reverse{flex-direction:column;}\n    .hx-intro .img,.hx-pair .img{flex:0 0 auto;width:100%;}\n    .hx-figure-grid,.hx-figure-grid.single{grid-template-columns:1fr;}\n    .hx-article h2{font-size:18px;}\n  }\n<\/style>\n<div class=\"hx-article\">\n<p>  <!-- ===== \ud83d\udcfa \u89c6\u9891\u94fe\u63a5\u7f6e\u9876 ===== --><\/p>\n<p class=\"hx-video-wrap\"><a class=\"hx-video-btn\" href=\"https:\/\/www.bilibili.com\/video\/BV1usEw6zEDS\/?vd_source=8d30165d99520ba73530f22c2aefc190\" target=\"_blank\" rel=\"noopener\">\ud83d\udcfa \u70b9\u51fb\u67e5\u770b\u89c6\u9891<\/a><\/p>\n<p>  <!-- ===== \u4ecb\u7ecd\u5361 ===== --><\/p>\n<div class=\"hx-intro\">\n<div class=\"info\">\n<div class=\"hx-callout info\" style=\"margin:0;\">\n        \ud83d\udd2c <b>\u5b9e\u9a8c\u540d\u79f0\uff1a<\/b>\u9ad8\u4fdd\u771f\u56fa\u5b9a\u6db2\u4e0e 4 \u79cd\u5c01\u7247\u5242\u5bf9\u5fae\u4e1d\u7ed3\u6784\u7ec6\u8282\u4fdd\u5b58\u7684\u5f71\u54cd<br \/>\n        \ud83c\udfaf <b>\u672c\u7ae0\u5185\u5bb9\uff1a<\/b>\u914d\u7f6e\u56fa\u5b9a\u6db2\n      <\/div>\n<p>\u672c\u7ae0\u8bb2\u89e3 <b>\u9ad8\u4fdd\u771f\u5fae\u4e1d\u56fa\u5b9a\u6db2<\/b> \u7684\u914d\u7f6e\u6d41\u7a0b\uff1a\u6837\u54c1\u5206\u7ec4\u3001\u5b9e\u9a8c\u7528\u5177\u3001\u914d\u7f6e\u8bd5\u5242\u6b65\u9aa4\u3001\u9884\u70ed\u5904\u7406\u3001\u64cd\u4f5c Tips\u3002<\/p>\n<\/p><\/div>\n<div class=\"img\">\n      <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1494\/%E5%9B%BA%E5%AE%9A%E6%B6%B2%E9%85%8D%E7%BD%AE.mp4_20260718_202400106.webp\" alt=\"\u5b9e\u9a8c\u6982\u51b5\">\n    <\/div>\n<\/p><\/div>\n<p>  <!-- ===== \u4e00\u3001\u5b9e\u9a8c\u6837\u54c1 ===== --><\/p>\n<h2>\u4e00\u3001\u5b9e\u9a8c\u6837\u54c1<\/h2>\n<div class=\"hx-figure-grid\">\n<figure class=\"hx-figure\">\n      <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1494\/%E5%9B%BA%E5%AE%9A%E6%B6%B2%E9%85%8D%E7%BD%AE.mp4_20260718_203905434.webp\" alt=\"\u6837\u54c1 1 \u5e38\u89c4\u56fa\u5b9a\u6db2\uff084%PFA\uff09\"><br \/>\n    <\/figure>\n<figure class=\"hx-figure\">\n      <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1494\/%E5%9B%BA%E5%AE%9A%E6%B6%B2%E9%85%8D%E7%BD%AE.mp4_20260718_202703094.webp\" alt=\"\u6837\u54c1 2-5 \u9ad8\u4fdd\u771f\u5fae\u4e1d\u56fa\u5b9a\u6db2\"><br \/>\n    <\/figure>\n<\/p><\/div>\n<div class=\"hx-callout key\">\n    \ud83e\uddea <b>\u6837\u54c1 1\uff1a<\/b>\u5e38\u89c4\u56fa\u5b9a\u6db2\uff08<b>4% PFA<\/b>\uff09\u4f5c\u4e3a\u5bf9\u7167\u7ec4\u57fa\u51c6\u53c2\u7167\u3002<br \/>\n    \ud83e\uddea <b>\u6837\u54c1 2-5\uff1a<\/b><b>\u9ad8\u4fdd\u771f\u5fae\u4e1d\u56fa\u5b9a\u6db2<\/b>\uff0c\u9488\u5bf9\u5fae\u4e1d\u7eb3\u7c73\u7ed3\u6784\u7ec6\u8282\u4e13\u95e8\u4f18\u5316\u3002\n  <\/div>\n<p>  <!-- ===== \u4e8c\u3001\u5b9e\u9a8c\u7528\u5177 ===== --><\/p>\n<h2>\u4e8c\u3001\u5b9e\u9a8c\u7528\u5177<\/h2>\n<div class=\"hx-figure-grid\">\n<figure class=\"hx-figure\">\n      <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1494\/%E5%9B%BA%E5%AE%9A%E6%B6%B2%E9%85%8D%E7%BD%AE.mp4_20260718_202552871.webp\" alt=\"3 \u652f\u79fb\u6db2\u67aa\"><br \/>\n    <\/figure>\n<figure class=\"hx-figure\">\n      <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1494\/%E5%9B%BA%E5%AE%9A%E6%B6%B2%E9%85%8D%E7%BD%AE.mp4_20260718_202607086.webp\" alt=\"5ml \u6ce8\u5c04\u5668 \u548c 2ml \u6ce8\u5c04\u5668\"><br \/>\n    <\/figure>\n<\/p><\/div>\n<ul class=\"hx-list\">\n<li><b>3 \u652f\u79fb\u6db2\u67aa<\/b>\uff08\u4e0d\u540c\u91cf\u7a0b\u914d\u5957\uff0c\u8986\u76d6\u53d6\u6db2\u5168\u6d41\u7a0b\uff09<\/li>\n<li><b>5 ml \u6ce8\u5c04\u5668 \u548c 2 ml \u6ce8\u5c04\u5668<\/b>\uff08\u7528\u4e8e\u5438\u53d6\u5fae\u4e1d\u56fa\u5b9a\u6db2\uff09<\/li>\n<\/ul>\n<div class=\"hx-figure-grid\">\n<figure class=\"hx-figure\">\n      <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1494\/%E5%9B%BA%E5%AE%9A%E6%B6%B2%E9%85%8D%E7%BD%AE.mp4_20260718_202627611.webp\" alt=\"\u954a\u5b50 + \u5438\u6db2\u7ba1\"><br \/>\n    <\/figure>\n<figure class=\"hx-figure\">\n      <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1494\/%E5%9B%BA%E5%AE%9A%E6%B6%B2%E9%85%8D%E7%BD%AE.mp4_20260718_202639903.webp\" alt=\"\u65e0\u83cc\u65e0\u5185\u6bd2\u7d20\u7684\u8d85\u7eaf\u5236\u6837 PBS\"><br \/>\n    <\/figure>\n<\/p><\/div>\n<ul class=\"hx-list\">\n<li><b>\u954a\u5b50<\/b>\uff08\u6216\u8005\u5176\u5b83\u786c\u7269\u9876\u5f00\u897f\u6797\u74f6\uff09<\/li>\n<li><b>\u5438\u6db2\u7ba1<\/b><\/li>\n<li><b>\u65e0\u83cc\u65e0\u5185\u6bd2\u7d20\u7684\u8d85\u7eaf\u5236\u6837 PBS<\/b><\/li>\n<\/ul>\n<p>  <!-- ===== \u4e09\u3001\u914d\u7f6e\u8bd5\u5242\u6b65\u9aa4 ===== --><\/p>\n<h2>\u4e09\u3001\u914d\u7f6e\u8bd5\u5242\u6b65\u9aa4<\/h2>\n<h3><span class=\"hx-badge\">\u2460<small>step<\/small><\/span>\u6807\u8bb0 control<\/h3>\n<p>\u7528\u9a6c\u514b\u7b14\u5728 <b>15 ml \u79bb\u5fc3\u7ba1<\/b> \u4e0a\u6807\u8bb0 <code>control<\/code> \u5b57\u6837\uff0c\u7ed9\u8be5 15 ml \u79bb\u5fc3\u7ba1\u4e2d\u6ce8\u5165 <b>2 ml \u5e38\u89c4\u56fa\u5b9a\u6db2<\/b>\u3002<\/p>\n<div class=\"hx-figure-grid\">\n<figure class=\"hx-figure\">\n      <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1494\/%E5%9B%BA%E5%AE%9A%E6%B6%B2%E9%85%8D%E7%BD%AE.mp4_20260718_203133040.webp\" alt=\"\u6807\u8bb0 control 1\"><br \/>\n    <\/figure>\n<figure class=\"hx-figure\">\n      <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1494\/%E5%9B%BA%E5%AE%9A%E6%B6%B2%E9%85%8D%E7%BD%AE.mp4_20260718_203147591.webp\" alt=\"\u6807\u8bb0 control 2\"><br \/>\n    <\/figure>\n<\/p><\/div>\n<h3><span class=\"hx-badge\">\u2461<small>step<\/small><\/span>\u6807\u8bb0 HF\uff08high fidelity\uff09<\/h3>\n<p>\u7528\u9a6c\u514b\u7b14\u5728\u8bd5\u7ba1\u4e0a\u6807\u8bb0 <code>HF (high fidelity)<\/code> \u5b57\u6837\uff0c\u5f00\u59cb\u914d\u7f6e\u9ad8\u4fdd\u771f\u5fae\u4e1d\u56fa\u5b9a\u6db2\u3002<\/p>\n<figure class=\"hx-figure\">\n    <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1494\/%E5%9B%BA%E5%AE%9A%E6%B6%B2%E9%85%8D%E7%BD%AE.mp4_20260718_202730831.webp\" alt=\"\u6807\u8bb0 HF\"><br \/>\n  <\/figure>\n<h4><span class=\"ico\">\ud83e\uddea<\/span>\u9ad8\u4fdd\u771f\u56fa\u5b9a\u6db2\u4e09\u79cd\u6210\u5206<\/h4>\n<ol class=\"hx-list\">\n<li><b>\u6210\u5206\u4e00\uff1a<\/b>Cell Actin Fixation Solution I &nbsp;&nbsp; <b>5 ml 16% \u8d85\u5206\u8fa8\u5149\u955c\u7ea7\u591a\u805a\u7532\u919b<\/b><\/li>\n<li><b>\u6210\u5206\u4e8c\uff1a<\/b>Cell Actin Fixation Solution II &nbsp;&nbsp; <b>40 \u00b5l 50% \u8d85\u5206\u8fa8\u5149\u955c\u7ea7\u620a\u4e8c\u919b<\/b><\/li>\n<li><b>\u6210\u5206\u4e09\uff1a<\/b>General Actin Buffer &nbsp;&nbsp; <b>5 ml<\/b>\uff08\u901a\u7528\u5fae\u4e1d\u7f13\u51b2\u6db2\uff09<\/li>\n<\/ol>\n<div class=\"hx-callout warn\">\n    <b>\u26a0\ufe0f \u6210\u5206\u4e09\u6ce8\u610f\u4e8b\u9879\uff1a<\/b><br \/>\n    \u2022 <b>-80\u2103<\/b> \u4fdd\u5b58\u6709\u6548\u671f\u7ea6 <b>12 \u4e2a\u6708<\/b><br \/>\n    \u2022 <b>-20\u2103<\/b> \u4fdd\u5b58\u65f6\u95f4\u4e3a <b>1-3 \u4e2a\u6708<\/b><br \/>\n    \u2022 <b>4\u2103 \u65e0\u6cd5\u4fdd\u5b58<\/b><br \/>\n    \u2022 \u6b63\u5e38\u8bd5\u5242\u89e3\u51bb\u540e\u5448\u73b0\u900f\u660e\u65e0\u8272\u6f84\u6e05\u7684\u72b6\u6001<br \/>\n    \u2022 \u274c \u53cd\u590d\u51bb\u5b58\u540e\u4f1a\u51fa\u73b0\u7816\u7ea2\u8272\u7684\u6c89\u6dc0\uff0c\u6b64\u65f6\u8bd5\u5242\u5c5e\u4e8e\u5931\u6548\u72b6\u6001\u5df2\u7ecf\u65e0\u6cd5\u4f7f\u7528<br \/>\n    \u2022 \ud83d\udca1 \u5c3d\u91cf\u4e00\u6b21\u7528\u5b8c\uff1b\u4e00\u6b21\u7528\u4e0d\u5b8c\uff0c\u5efa\u8bae\u5206\u88c5\u540e <b>-80\u2103<\/b> \u4fdd\u5b58\uff0c\u968f\u7528\u968f\u53d6\u89e3\u51bb\u540e\u4f7f\u7528\u3002\n  <\/div>\n<figure class=\"hx-figure\">\n    <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1494\/image.webp\" alt=\"\u4e09\u79cd\u6210\u5206\u74f6\"><br \/>\n  <\/figure>\n<h4><span class=\"ico\">\ud83e\uddee<\/span>\u7528\u91cf\u8ba1\u7b97<\/h4>\n<p>\u653e\u7f6e\u4e86\u7ec6\u80de\u722c\u7247\u7684\u5851\u6599 <b>3.5 mm \u5c0f\u76bf<\/b>\uff1a\u9700\u8981 <b>2 ml \u5de5\u4f5c\u6db2<\/b>\u624d\u80fd\u5b8c\u5168\u8986\u76d6\u722c\u7247\u3002<\/p>\n<div class=\"hx-callout key\">\n    <b>\u5de5\u4f5c\u6db2\u914d\u6bd4\uff1a<\/b><br \/>\n    \u2022 <b>1 ml \u6210\u5206\u4e00<\/b> Cell Actin Fixation Solution I<br \/>\n    \u2022 <b>1 ml \u6210\u5206\u4e09<\/b> General Actin Buffer<br \/>\n    \u2022 \u914d <b>8 \u00b5l \u6210\u5206\u4e8c<\/b> Cell Actin Fixation Solution II<\/p>\n<p>    \u6bd4\u4f8b\u4e3a <b>0.5 ml \uff1a0.5 ml\uff1a4 \u00b5l<\/b><br \/>\n    \u5373 <b>1 ml<\/b> \u6210\u5206\u4e00\u548c\u6210\u5206\u4e09 \u6df7\u5408\u6db2\uff0c\u914d\u6bd4 <b>8 \u00b5l<\/b> \u6210\u5206\u4e8c\u3002<br \/>\n    \u672c\u6b21\u5b9e\u9a8c\u6bcf\u4e2a\u5c0f\u76bf\uff0c\u5404\u9700\u8981 <b>2.08 ml\uff08\u7ea6 2 ml\uff09<\/b> \u56fa\u5b9a\u5de5\u4f5c\u6db2\u3002<br \/>\n    \u4e3a\u914d\u6bd4\u8db3\u591f\u5236\u5907 <b>5 \u4e2a\u5c0f\u76bf<\/b> \u7684\u56fa\u5b9a\u5de5\u4f5c\u6db2\uff0c\u914d\u7f6e <b>5 \u500d\u5de5\u4f5c\u6db2<\/b>\uff0c\u5373\uff1a<b>5 ml\uff1a5 ml\uff1a40 \u00b5l<\/b>\u3002\n  <\/div>\n<figure class=\"hx-figure\">\n    <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1494\/%E5%9B%BA%E5%AE%9A%E6%B6%B2%E9%85%8D%E7%BD%AE.mp4_20260718_202747925.webp\" alt=\"\u7528\u91cf\u8ba1\u7b97\u793a\u610f\"><br \/>\n  <\/figure>\n<h4><span class=\"ico\">\ud83d\udd27<\/span>\u914d\u7f6e\u6b65\u9aa4<\/h4>\n<div class=\"hx-figure-grid\">\n<figure class=\"hx-figure\">\n      <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1494\/%E5%9B%BA%E5%AE%9A%E6%B6%B2%E9%85%8D%E7%BD%AE.mp4_20260718_202835324.webp\" alt=\"\u5438\u51fa\u6210\u5206\u4e00\"><br \/>\n    <\/figure>\n<figure class=\"hx-figure\">\n      <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1494\/%E5%9B%BA%E5%AE%9A%E6%B6%B2%E9%85%8D%E7%BD%AE.mp4_20260718_211700537.webp\" alt=\"\u5438\u51fa\u6210\u5206\u4e09\"><br \/>\n    <\/figure>\n<\/p><\/div>\n<p><span class=\"hx-badge\">1<small>step<\/small><\/span>\u5438\u51fa\u6210\u5206\u4e00 Cell Actin Fixation Solution I <b>5 ml<\/b><\/p>\n<p><span class=\"hx-badge\">2<small>step<\/small><\/span>\u5438\u51fa\u6210\u5206\u4e09 General Actin Buffer <b>5 ml<\/b><\/p>\n<div class=\"hx-figure-grid\">\n<figure class=\"hx-figure\">\n      <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1494\/%E5%9B%BA%E5%AE%9A%E6%B6%B2%E9%85%8D%E7%BD%AE.mp4_20260718_211754053.webp\" alt=\"\u5438\u51fa\u6210\u5206\u4e8c\"><br \/>\n    <\/figure>\n<figure class=\"hx-figure\">\n      <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1494\/%E5%9B%BA%E5%AE%9A%E6%B6%B2%E9%85%8D%E7%BD%AE.mp4_20260718_211851979.webp\" alt=\"\u6df7\u5408\u6eb6\u6db2\"><br \/>\n    <\/figure>\n<\/p><\/div>\n<p><span class=\"hx-badge\">3<small>step<\/small><\/span>\u5438\u51fa\u6210\u5206\u4e8c Cell Actin Fixation Solution II <b>40 \u00b5l<\/b><\/p>\n<p><span class=\"hx-badge\">4<small>step<\/small><\/span><b>\u6df7\u5408\u6eb6\u6db2<\/b> \u2705<\/p>\n<p>  <!-- ===== \u56db\u3001\u9884\u70ed\u5904\u7406 ===== --><\/p>\n<h2>\u56db\u3001\u9884\u70ed\u5904\u7406<\/h2>\n<p><span class=\"hx-badge\">\u2462<small>step<\/small><\/span>\u914d\u7f6e\u5b8c\u6210\u7684 <b>\u9ad8\u4fdd\u771f\u56fa\u5b9a\u6db2<\/b>\uff08\u5305\u62ec\u666e\u901a\u56fa\u5b9a\u6db2\uff09\u548c<b>\u5c01\u95ed\u6253\u5b54\u6db2<\/b>\u4e00\u5b9a\u8981\u9884\u70ed\u5230 <b>37.5\u2103<\/b>\u3002<\/p>\n<div class=\"hx-callout info\" style=\"margin:0 0 16px;\">\n    \ud83d\udca1 \u56fe\u4e2d\u9884\u70ed\u5230 <b>38.5\u2103<\/b> \u662f\u56e0\u4e3a\u5ba4\u6e29\u6bd4\u8f83\u4f4e\uff0c\u6240\u4ee5\u7a0d\u5fae\u4e0a\u8c03\u4e86\u4e00\u4e9b\u3002\n  <\/div>\n<p><b>\u5c01\u95ed\u6253\u5b54\u6db2\u662f\u63d0\u524d\u914d\u597d\u7684<\/b>\uff0c\u6210\u5206\uff1a<\/p>\n<table class=\"hx-table\">\n<thead>\n<tr>\n<th style=\"width:8%;\">#<\/th>\n<th>\u540d\u79f0<\/th>\n<th style=\"width:12%;\">\u89c4\u683c<\/th>\n<th style=\"width:8%;\">\u6570\u91cf<\/th>\n<th style=\"width:18%;\">\u50a8\u5b58<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>1<\/td>\n<td>Sunbloss\u00ae Blocking &amp; Permeabilization Solution <b>I<\/b><\/td>\n<td><b>0.5 ml<\/b><\/td>\n<td>36<\/td>\n<td>-20 \u5ea6\u50a8\u5b58<\/td>\n<\/tr>\n<tr>\n<td>2<\/td>\n<td>Sunbloss\u00ae Blocking &amp; Permeabilization Solution <b>II<\/b><\/td>\n<td><b>1.8 ml<\/b><\/td>\n<td>1<\/td>\n<td>\u5ba4\u6e29 25 \u5ea6\u50a8\u5b58<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<figure class=\"hx-figure\">\n    <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/1494\/%E5%9B%BA%E5%AE%9A%E6%B6%B2%E9%85%8D%E7%BD%AE.mp4_20260718_203018181.webp\" alt=\"\u9884\u70ed\u5c01\u95ed\u6253\u5b54\u6db2\"><br \/>\n  <\/figure>\n<p>  <!-- ===== \u4e94\u3001\u64cd\u4f5c Tips ===== --><\/p>\n<h2>\u4e94\u3001\u64cd\u4f5c Tips<\/h2>\n<h4><span class=\"ico\">\ud83e\uddea<\/span>\u897f\u6797\u74f6\u6253\u5f00\u6280\u5de7<\/h4>\n<p>\u6309\u7167\u7bad\u5934\u6307\u793a\u7528\u786c\u7269<b>\u4ece\u4e0b\u5f80\u4e0a\u9876\u5f00<\/b>\uff0c\u94dd\u7b94\u7247\u81ea\u7136\u4f1a\u88ab\u5e26\u8d77\u3002<\/p>\n<h4><span class=\"ico\">\ud83d\udc89<\/span>\u897f\u6797\u74f6\u53d6\u8bd5\u5242\u6280\u5de7<\/h4>\n<ul class=\"hx-list\">\n<li>\u4ece\u4e2d\u95f4\u5b54\u63d2\u8fdb\u53bb\uff0c\u6709<b>\u8d1f\u538b<\/b>\uff0c\u8981\u7528\u5927\u62c7\u6307\u7528\u70b9\u529b\u53bb\u63a8\u9488\u7ba1\u3002<\/li>\n<li>\u628a\u897f\u6797\u74f6<b>\u5012\u6263\u8fc7\u6765<\/b>\uff0c\u9488\u5934\u5c3d\u91cf\u4e0d\u8981\u5438\u5230\u7a7a\u6c14\uff0c\u5438\u5230\u7a7a\u6c14\u5c31\u628a\u9488\u5934\u5411\u4e0a\uff0c\u628a\u7a7a\u6c14\u6253\u56de\u53bb\u3002<\/li>\n<\/ul>\n<h4><span class=\"ico\">\ud83d\udee1\ufe0f<\/span>\u6ce8\u5c04\u5668\u4f7f\u7528\u5b89\u5168<\/h4>\n<ul class=\"hx-list\">\n<li>\u6ce8\u5c04\u5668\u7528\u5b8c\u8981<b>\u76d6\u4e0a\u76d6\u5b50<\/b>\u518d\u53bb\u6389\u3002<\/li>\n<li>\u6bcf\u652f\u6ce8\u5c04\u5668\u53ea\u5438\u53d6\u4e00\u79cd\u6db2\u4f53\uff0c\u53d6\u5176\u4ed6 buffer \u8bf7\u66f4\u6362\u6ce8\u5c04\u5668\u3002<\/li>\n<\/ul>\n<h4><span class=\"ico\">\ud83d\udcd0<\/span>\u8bf7\u6ce8\u610f\u5de5\u4f5c\u6db2\u5145\u5206\u6d78\u6ca1\u7ec6\u80de<\/h4>\n<table class=\"hx-table\">\n<thead>\n<tr>\n<th>\u5c0f\u76bf\u7c7b\u578b<\/th>\n<th style=\"width:40%;\">\u6240\u9700\u5de5\u4f5c\u6db2<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td><b>3.5 mm \u5171\u805a\u7126\u73bb\u5e95\u5c0f\u76bf<\/b>\uff08\u5e26\u51f9\u69fd\uff09<\/td>\n<td>\u51f9\u69fd\u4e2d\u9700\u8981 <b>500 \u00b5l<\/b> \u5de5\u4f5c\u6db2\u4ee5\u4e0a\u5c31\u53ef\u4ee5\u5145\u5206\u6d78\u6ca1<\/td>\n<\/tr>\n<tr>\n<td><b>3.5 mm \u5851\u6599\u5e95\u5c0f\u76bf<\/b> \uff0b \u7ec6\u80de\u722c\u7247<\/td>\n<td>\u9700\u8981 <b>2 ml<\/b> \u5de5\u4f5c\u6db2\u624d\u80fd\u5b8c\u5168\u5305\u88f9\u8986\u76d6\u722c\u7247<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<div class=\"hx-callout warn\" style=\"margin:0 0 16px;\">\n    \u26a0\ufe0f \u56fa\u5b9a\u6db2\u9700\u8981\u9884\u70ed\u5230 <b>37 \u5ea6<\/b>\uff0c\u8bf7\u7528\u91d1\u5c5e\u6d74\u6216\u6c34\u6d74\u9505\u5b9e\u73b0\u9884\u70ed\u3002\n  <\/div>\n<p class=\"hx-end\">\u2014\u2014 \u9ad8\u4fdd\u771f\u5fae\u4e1d\u56fa\u5b9a \u00b7 \u56fe\u6587\u5b9e\u9a8c\u6d41\u7a0b\uff08\u4e00\uff09\u2014\u2014<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u5b9e\u9a8c\u540d\u79f0\uff1a\u9ad8\u4fdd\u771f\u56fa\u5b9a\u6db2\u4e0e 4 \u79cd\u5c01\u7247\u5242\u5bf9\u5fae\u4e1d\u7ed3\u6784\u7ec6\u8282\u4fdd\u5b58\u7684\u5f71\u54cd<br \/>\n\u672c\u7ae0\u8282\u7684\u4e3b\u8981\u5185\u5bb9\uff1a\u914d\u7f6e\u56fa\u5b9a\u6db2<\/p>\n<p>[\u56fe\u7247]<br \/>\n\u6837\u54c11\uff1a\u5e38\u89c4\u56fa\u5b9a\u6db2\uff084%PFA\uff09<\/p>\n<p>[\u56fe\u7247]<br \/>\n\u6837\u54c12-\u6837\u54c1 5\uff1a\u9ad8\u4fdd\u771f\u5fae\u4e1d\u56fa\u5b9a\u6db2<br \/>\n[\u56fe\u7247]<\/p>\n<p>\u5b9e\u9a8c\u7528\u5177\uff1a<br \/>\n3\u652f\u79fb\u6db2\u67aa<br \/>\n[\u56fe\u7247]<br \/>\n5ml\u6ce8\u5c04\u5668 \u548c 2ml \u6ce8\u5c04\u5668\uff08\u53d6\u5fae\u4e1d\u56fa\u5b9a\u6db2\uff09<br \/>\n[\u56fe\u7247]<\/p>\n<p>\u954a\u5b50\uff08\u6216\u8005\u5176\u5b83\u786c\u7269\u9876\u5f00\u897f\u6797\u74f6\uff09<br \/>\n\u5438\u6db2\u7ba1<br \/>\n[\u56fe\u7247]<\/p>\n<p>\u65e0\u83cc\u65e0\u5185\u6bd2\u7d20\u7684\u8d85\u7eaf\u5236\u6837PBS<\/p>\n<p>[\u56fe\u7247]<\/p>\n<p>\u914d\u7f6e\u8bd5\u5242\u6b65\u9aa4<br \/>\n\u2460 \u7528\u9a6c\u514b\u7b14\u572815ml \u79bb\u5fc3\u7ba1\u4e0a\u6807\u8bb0control\u5b57\u6837\uff0c\u7ed9\u8be515ml\u79bb\u5fc3\u7ba1\u4e2d\u6ce8\u51652ml\u5e38\u89c4\u56fa\u5b9a\u6db2\u3002<\/p>\n<p>[\u56fe\u7247]<br \/>\n[\u56fe\u7247]<\/p>\n<p>\u2461 \u7528\u9a6c\u514b\u7b14\u5728\u8bd5\u7ba1\u4e0a\u6807\u8bb0HF(high fidelity)\u5b57\u6837\uff0c\u5f00\u59cb\u914d\u7f6e\u9ad8\u4fdd\u771f\u5fae\u4e1d\u56fa\u5b9a\u6db2\u3002<br \/>\n[\u56fe\u7247]<br \/>\n\u9ad8\u4fdd\u771f\u56fa\u5b9a\u6db2\u4e09\u79cd\u6210\u5206\uff1a<br \/>\n1. \u6210\u5206\u4e00\uff1aCell Actin Fixation Solution I<br \/>\n      5ml 16%\u8d85\u5206\u8fa8\u5149\u955c\u7ea7\u591a\u805a\u7532\u919b<br \/>\n2. \u6210\u5206\u4e8c\uff1aCell Actin Fixation Solution II<br \/>\n      40\u00b5l 50%\u8d85\u5206\u8fa8\u5149\u955c\u7ea7\u620a\u4e8c\u919b<br \/>\n3. \u6210\u5206\u4e09\uff1aGeneral Actin Buffer<br \/>\n      5ml  \uff08\u901a\u7528\u5fae\u4e1d\u7f13\u51b2\u6db2\uff09<br 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