{"id":1322,"date":"2026-07-23T14:48:10","date_gmt":"2026-07-23T06:48:10","guid":{"rendered":"https:\/\/www.hxicollege.com\/?p=1322"},"modified":"2026-07-28T14:48:00","modified_gmt":"2026-07-28T06:48:00","slug":"case-01-sample-3d-confocal-1322","status":"publish","type":"post","link":"https:\/\/www.hxicollege.com\/index.php\/2026\/07\/23\/case-01-sample-3d-confocal-1322\/","title":{"rendered":"\u6848\u4f8b 01\uff1a\u9ad8\u4fdd\u771f\u8d85\u5206\u8fa8\u6837\u672c-\u7ec6\u80de\u5fae\u7ba13D\u5171\u805a\u7126\u6210\u50cf"},"content":{"rendered":"<style>\n  \/* ===== \u6574\u7bc7\u5bb9\u5668 ===== *\/\n  .hx-article{\n    max-width:1080px;\n    margin:0 auto;\n    padding:40px 28px 64px;\n    font-family:-apple-system,BlinkMacSystemFont,\"Segoe UI\",\"PingFang SC\",\"Hiragino Sans GB\",\"Microsoft YaHei\",sans-serif;\n    color:#1f2328;\n    line-height:1.85;\n    font-size:16px;\n  }\n  \/* ===== h2 \u6848\u4f8b\u6807\u9898\uff08h1 \u7531 post_title \u6e32\u67d3\uff09 ===== *\/\n  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\/* ===== CelebNews \u591c\u95f4\u6a21\u5f0f\u9002\u914d ===== *\/\n  body.aft-dark-mode .hx-article,\n  body.aft-dark-mode .hx-article p{color:#e2e8f0;}\n  body.aft-dark-mode .hx-article h2{color:#f1f5f9;}\n  \/* hx-meta \u6697\u8272\u89c4\u5219\u5df2\u5220\u9664\uff08\u654f\u611f\u4fe1\u606f\uff09 *\/\n  body.aft-dark-mode .hx-figure{background:#1e293b;box-shadow:0 1px 3px rgba(0,0,0,.3),0 0 0 1px #334155;}\n  body.aft-dark-mode .hx-protocol{background:#1e293b;border-color:#334155;}\n  body.aft-dark-mode .hx-protocol .p-title{color:#93c5fd;}\n  body.aft-dark-mode .hx-protocol li{color:#cbd5e1;}\n  body.aft-dark-mode .hx-protocol li::before{color:#fb923c;}\n  body.aft-dark-mode .hx-protocol .k{background:rgba(37,99,235,.25);color:#93c5fd;}\n  body.aft-dark-mode .hx-block h4{color:#f1f5f9;}\n  body.aft-dark-mode .hx-block p,\n  body.aft-dark-mode .hx-block ol,\n  body.aft-dark-mode .hx-block ol li{color:#cbd5e1;}\n  body.aft-dark-mode .hx-block .info-line{color:#cbd5e1;}\n  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<!-- ===== \u5de6\u680f\uff1a\u56fe + Protocol ===== --><\/p>\n<div class=\"hx-col\">\n<figure class=\"hx-figure\">\n        <img decoding=\"async\" src=\"https:\/\/www.hxicollege.com\/wp-content\/uploads\/2026\/07\/2026720\u76f4\u64adcase1.png\" alt=\"\u7ec6\u80de\u5fae\u7ba13D\u5171\u805a\u7126\u6210\u50cf\"><br \/>\n      <\/figure>\n<div class=\"hx-protocol\" style=\"margin-top:18px;\">\n<p class=\"p-title\"><span class=\"ico\">\ud83e\uddea<\/span><span>Protocol for high-fidelity Fixed 3D Confocal Sample<\/span><\/p>\n<ol>\n<li>\u9ad8\u4fdd\u771f\u5fae\u7ba1\u56fa\u5b9a\uff0c\u4f7f\u7528<span class=\"k\">\u9ad8\u4fdd\u771f\u56fa\u5b9a\u6db2-\u5fae\u7ba1<\/span>37 \u2103\u56fa\u5b9a\u7ec6\u80de 10min\uff1b<\/li>\n<li>\u4f7f\u75282ml <span class=\"k\">\u8d85\u7eaf\u5236\u6837PBS<\/span>\u7b80\u77ed\u7684\u6e05\u6d17\u7ec6\u80de 3 \u6b21\uff1b<\/li>\n<li>\u538b\u5236\u80cc\u666f\u8367\u5149\uff0c\u4f7f\u75282ml\u914d\u7f6e\u597d\u7684 <span class=\"k\">\u81ea\u53d1\u8367\u5149\u6291\u5236\u5242<\/span>\uff0c\u5728\u5ba4\u6e29\u4e0b\u5b75\u80b2\u6837\u672c 7min\uff0c\u4f7f\u7528\u4fa7\u6446\u6447\u5e8a\u8f85\u52a9\u4ea4\u6362\u6db2\u4f53\uff1b<\/li>\n<li>\u4f7f\u75282ml <span class=\"k\">\u8d85\u7eaf\u5236\u6837PBS<\/span>\u7b80\u77ed\u7684\u6e05\u6d17\u7ec6\u80de 3 \u6b21\uff1b<\/li>\n<li>\u5c01\u95ed\u6297\u4f53\u975e\u7279\u5f02\u6027\u7ed3\u5408\u4f4d\u70b9\uff0c\u4f7f\u7528 50ul \u914d\u7f6e\u597d\u7684<span class=\"k\">\u5c01\u95ed\u6253\u5b54\u6db2<\/span>\uff0c\u540c\u65f6\u4f7f\u7528 <span class=\"k\">\u5236\u6837\u4f34\u4fa3-\u8d34\u58c1\u6837\u672c<\/span>\u8f85\u52a9\u6837\u672c\u7684\u5b75\u80b2\uff0c\u5ba4\u6e29\u4e0b\u5b75\u80b2\u7ec6\u80de 30 min\uff1b<\/li>\n<li>\u4f7f\u7528 <span class=\"k\">\u5236\u6837\u4f34\u4fa3-\u8d34\u58c1\u6837\u672c<\/span>\u8f85\u52a9\u4e00\u6297\u5b75\u80b2\uff0c\u4fdd\u6301\u6e7f\u6da6\u73af\u5883\uff0c\u5ba4\u6e29\u5b75\u80b2 1 \u5c0f\u65f6\u3002\u4e00\u6297\u9009\u62e9 beta-tubulin E7 mouse\uff1b<\/li>\n<li>\u4f7f\u7528 2ml <span class=\"k\">\u8d85\u7eaf\u5236\u6837PBS<\/span>\u6e05\u6d17\u4e00\u6297\uff0c5min \u4e00\u6b21\uff0c\u6e05\u6d173\u6b21\uff0c\u4f7f\u7528\u4fa7\u6446\u6447\u5e8a\u8f85\u52a9\u4ea4\u6362\u6db2\u4f53\uff1b<\/li>\n<li>\u62b5\u5fa1\u975e\u7279\u5f02\u6027\u8367\u5149\u67d3\u6599\u7ed3\u5408\uff0c\u4f7f\u752825ul <span class=\"k\">\u8367\u5149\u51c0\u5316\u589e\u5f3a\u6db2<\/span>\uff0c\u540c\u65f6\u4f7f\u7528 <span class=\"k\">\u5236\u6837\u4f34\u4fa3-\u8d34\u58c1\u6837\u672c<\/span>\u8f85\u52a9\u6837\u672c\u7684\u5b75\u80b2\uff0c\u5ba4\u6e29\u5b75\u80b2\u6837\u672c 30min\uff1b<\/li>\n<li>\u4f7f\u7528 <span class=\"k\">\u5236\u6837\u4f34\u4fa3-\u8d34\u58c1\u6837\u672c<\/span>\u8f85\u52a9\u4e8c\u6297\u5b75\u80b2\u3002\u4fdd\u6301\u6e7f\u6da6\u73af\u5883\uff0c\u5ba4\u6e29\u5b75\u80b2 1 \u5c0f\u65f6\u3002\u4e8c\u6297\u9009\u62e9 Alexa555 goat-anti-mouse IgG\u3002<\/li>\n<li>\u4f7f\u7528 2ml <span class=\"k\">\u8d85\u7eaf\u5236\u6837PBS<\/span>\u6e05\u6d17\u4e8c\u6297\uff0c5min \u4e00\u6b21\uff0c\u6e05\u6d173\u6b21\uff0c\u4f7f\u7528\u4fa7\u6446\u6447\u5e8a\u8f85\u52a9\u4ea4\u6362\u6db2\u4f53\uff1b<\/li>\n<li>\u4f7f\u7528 <span class=\"k\">\u8d85\u5206\u8fa8\u5149\u955c\u7ea7PFA-16%<\/span>\uff0c\u9884\u5236\u6210 4%\u6d53\u5ea6\uff0c\u53d6 500ul \u6d78\u6ca1\u6837\u54c1\u8fdb\u884c\u540e\u56fa\u5b9a\uff0c\u65f6\u95f410min\u3002<\/li>\n<li>\u5728\u6210\u50cf\u65f6\uff0c\u4f7f\u7528<span class=\"k\">Antifade\u6210\u50cf\u7f13\u51b2\u6db2<\/span>\uff0c\u8fdb\u884c 3D Confocal\u6210\u50cf\u3002<\/li>\n<\/ol><\/div>\n<\/p><\/div>\n<p>    <!-- ===== \u53f3\u680f\uff1a\u2b50 \u89e3\u8bfb ===== --><\/p>\n<div class=\"hx-col\">\n<div class=\"hx-block\">\n<h4>\u2b50 \u56fe\u50cf\u63cf\u8ff0\uff1a<\/h4>\n<p>\u6574\u4e2a\u7ec6\u80de\u5448\u73b0\u51fa\u5706\u6247\u5f62\uff0c\u6241\u5e73\u5730\u94fa\u5c55\u5728\u89c6\u91ce\u4e2d\uff0c\u7ec6\u80de\u5185\u7684\u5fae\u7ba1\u5448\u8f90\u5c04\u72b6\u6392\u5217\uff0c\u8fde\u7eed\u3001\u5747\u5300\u3001\u660e\u4eae\u3002\u8fd9\u662f\u4e00\u5f203D\u6210\u50cf\u76842D\u6295\u5f71\u56fe\uff0c\u901a\u8fc7\u989c\u8272\u4fe1\u606f\u533a\u5206\u9ad8\u5ea6\uff0c\u7070\u8272\u4ee3\u8868\u7ec6\u80de\u8f83\u4f4e\u7684\u533a\u57df\uff0c\u6a58\u8272\u4ee3\u8868\u8f83\u9ad8\u7684\u533a\u57df\u3002<\/p>\n<\/p><\/div>\n<div class=\"hx-block\">\n<h4>\u2b50 \u6807\u8bb0\u8981\u70b9\uff1a<\/h4>\n<ol>\n<li>\u57f9\u517b\u8d34\u58c1\u826f\u597d\u3001\u9ad8\u6d3b\u529b\u4e14\u7ec6\u80de\u4f53\u6241\u5e73\u7684\u7ec6\u80de\u3002<\/li>\n<li>\u5fae\u7ba1\u662f\u5bf9\u6e29\u5ea6\u6781\u5176\u654f\u611f\u7684\u7ec6\u80de\u5668\u3002\u4ece\u57f9\u517b\u7bb1\u53d6\u51fa\u5230\u56fa\u5b9a\u7684\u5168\u8fc7\u7a0b\uff0c\u5fc5\u987b\u4e25\u683c\u907f\u514d\u6e29\u5ea6\u6ce2\u52a8\u3002<\/li>\n<li>\u9009\u7528\u9002\u5408\u5fae\u7ba1\u7684\u9ad8\u4fdd\u771f\u56fa\u5b9a\u6db2\uff0c\u9632\u6b62\u5fae\u7ba1\u89e3\u805a\u3002<\/li>\n<li>\u4e3a\u4f7f\u80cc\u666f\u66f4\u5e72\u51c0\uff0c\u4f7f\u7528Quench\u8bd5\u5242\u6765\u6291\u5236\u81ea\u53d1\u8367\u5149\u3002<\/li>\n<li>\u4f7f\u7528\u6bd4\u5e38\u89c4\u63a8\u8350\u6d53\u5ea6\u66f4\u9ad8\u7684\u4e00\u6297\u6d53\u5ea6\u53bb\u8fdb\u884c\u8fc7\u9971\u548c\u6807\u8bb0\u3002<\/li>\n<\/ol><\/div>\n<div class=\"hx-block\">\n<h4>\u2b50 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class=\"info-line\"><b>\u4e00\u6297\uff1a<\/b>beta tubulin E7<\/p>\n<p class=\"info-line\"><b>\u4e8c\u6297\uff1a<\/b>Alexa555<\/p>\n<\/p><\/div>\n<div class=\"hx-block\">\n<h4>\u2b50 \u6210\u50cf\u6a21\u5f0f\uff1a<\/h4>\n<p>3D confocal \u6a21\u5f0f\uff0cOlympus FV3000 \u70b9\u626b\u63cf\u5171\u805a\u7126\u663e\u5fae\u955c\uff1b\u539f\u59cb\u56fe\u50cf\u7ecf\u8fc7\u4e86 3D \u53bb\u5377\u79ef\uff08Deconvolution\uff09\u5904\u7406\uff0c\u4f7f\u5fae\u7ba1\u7ea4\u7ef4\u770b\u8d77\u6765\u66f4\u52a0\u5e72\u51c0\u3001\u8fde\u7eed\u3002<\/p>\n<\/p><\/div>\n<div class=\"hx-block\">\n<h4>\u2b50 \u603b\u7ed3\uff1a<\/h4>\n<p>\u4e00\u5f20&#8221;\u6ee1\u5206&#8221;\u7684\u5fae\u7ba1\u56fe = \u5b8c\u7f8e\u7684\u6241\u5e73\u7ec6\u80de + \u4e25\u683c\u7684\u6e29\u63a7\u9ad8\u4fdd\u771f\u56fa\u5b9a + \u8fc7\u9971\u548c\u6807\u8bb0 + \u7cbe\u51c6\u7684\u89c6\u91ce\u6311\u9009 + \u5c0f\u95f4\u8ddd Z \u5c42\u626b + 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